ArticleInvestigative ophthalmology & visual science2015
Proteomic Analysis of Embryonic and Young Human Vitreous.
Article in Investigative ophthalmology & visual science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Identification of Potential Growth-Related Proteins in Chick Vitreous during Emmetropization Using SWATH-MS and Targeted-Based Proteomics (MRMHR).International journal of molecular sciences · 2024Article
- System-wide vitreous proteome dissection reveals impaired sheddase activity in diabetic retinopathy.Theranostics · 2022Article
- Diffusion and Protein Corona Formation of Lipid-Based Nanoparticles in the Vitreous Humor: Profiling and Pharmacokinetic Considerations.Molecular pharmaceutics · 2021Article
- Vitreous Antioxidants, Degeneration, and Vitreo-Retinopathy: Exploring the Links.Antioxidants (Basel, Switzerland) · 2019Review
- Simultaneous Improvement in the Precision, Accuracy, and Robustness of Label-free Proteome Quantification by Optimizing Data Manipulation Chains.Molecular & cellular proteomics : MCP · 2019Article
- The Human Eye Proteome Project: Updates on an Emerging Proteome.Proteomics · 2018Review
- Proteomic profiling of human intraschisis cavity fluid.Clinical proteomics · 2017Article
- Proteomic Analysis of the Bovine and Human Ciliary Zonule.Investigative ophthalmology & visual science · 2017Article
- Differential Expression of Vitreous Proteins in Young and Mature New Zealand White Rabbits.PloS one · 2016Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
purposeThe proteomic profile of vitreous from second-trimester human embryos and young adults was characterized using mass spectrometry and analyzed for changes in protein levels that may relate to structural changes occurring during this time. This vitreous proteome was compared to previous reports to confirm proteins already identified and reveal novel ones.
methodsVitreous from 17 human embryos aged 14 to 20 weeks gestation (WG) and from a 12-, a 14-, a 15-, and a 28-year-old was individually analyzed using tandem mass spectrometry-based proteomics. Peptide spectral count associations with embryonic age were assessed using a general linear model of fold changes and Spearman's rank correlation. Differences between embryonic and young adult vitreous proteomes were also compared. Immunohistochemistry was used to evaluate three proteins in five additional fetal (10-18 WG) human eyes.
resultsThere were 1217 proteins identified in fetal and young adult human vitreous, 206 after quantile normalization and variance filtering. In embryos, the peptide counts of 37 proteins changed significantly from 14 to 20 WG: 75.7% increased, 24.3% decreased. Immunohistochemistry confirmed the absence of clusterin and cadherin in 10 and 14 WG eyes and their presence at 18 WG. Comparing embryonic to young adult vitreous, 47 proteins were significantly higher or lower. A total of 768 proteins not previously identified in the literature are presented.
conclusionsProteins previously unreported in the human vitreous were identified. The human vitreous proteome undergoes significant changes during embryogenesis and young adulthood. A number of protein levels change considerably during the second trimester, with the majority decreasing.
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