ArticlePloS one2015
13C- and 15N-Labeling Strategies Combined with Mass Spectrometry Comprehensively Quantify Phospholipid Dynamics in C. elegans.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- LIPL-1 and LIPL-2 are TCER-1-regulated lysosomal lipases with distinct roles in immunity and fertility.PLoS genetics · 2025Article
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- An inducible and reversible system to regulate unsaturated fatty acid biosynthesis in C. elegans.G3 (Bethesda, Md.) · 2025Article
- Oleic Acid Metabolism in Response to Glucose inMetabolites · 2023Article
- Article
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- AdipoR2 recruits protein interactors to promote fatty acid elongation and membrane fluidity.The Journal of biological chemistry · 2023Article
- Bacterial diet modulates tamoxifen-induced death via host fatty acid metabolism.Nature communications · 2022Article
- Regulation and functions of membrane lipids: Insights fromBBA advances · 2022Review
- Quo VadisMetabolites · 2021Review
- Worms, Fat, and Death:Metabolites · 2021Review
- Potential Role of Plasmalogens in the Modulation of Biomembrane Morphology.Frontiers in cell and developmental biology · 2021Review
- Membrane fluidity is regulated by theeLife · 2018Article
- Membrane Fluidity Is Regulated Cell Nonautonomously byGenetics · 2018Article
- FRAP: A Powerful Method to Evaluate Membrane Fluidity inBio-protocol · 2018Article
- The biotin-ligating protein BPL-1 is critical for lipid biosynthesis and polarization of theThe Journal of biological chemistry · 2018Article
- Lipid and Carbohydrate Metabolism inGenetics · 2017Review
- The adiponectin receptor AdipoR2 and its Caenorhabditis elegans homolog PAQR-2 prevent membrane rigidification by exogenous saturated fatty acids.PLoS genetics · 2017Article
- Caenorhabditis elegans PAQR-2 and IGLR-2 Protect against Glucose Toxicity by Modulating Membrane Lipid Composition.PLoS genetics · 2016Article
- Using Caenorhabditis elegans to Uncover Conserved Functions of Omega-3 and Omega-6 Fatty Acids.Journal of clinical medicine · 2016Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Membranes define cellular and organelle boundaries, a function that is critical to all living systems. Like other biomolecules, membrane lipids are dynamically maintained, but current methods are extremely limited for monitoring lipid dynamics in living animals. We developed novel strategies in C. elegans combining 13C and 15N stable isotopes with mass spectrometry to directly quantify the replenishment rates of the individual fatty acids and intact phospholipids of the membrane. Using multiple measurements of phospholipid dynamics, we found that the phospholipid pools are replaced rapidly and at rates nearly double the turnover measured for neutral lipid populations. In fact, our analysis shows that the majority of membrane lipids are replaced each day. Furthermore, we found that stearoyl-CoA desaturases (SCDs), critical enzymes in polyunsaturated fatty acid production, play an unexpected role in influencing the overall rates of membrane maintenance as SCD depletion affected the turnover of nearly all membrane lipids. Additionally, the compromised membrane maintenance as defined by LC-MS/MS with SCD RNAi resulted in active phospholipid remodeling that we predict is critical to alleviate the impact of reduced membrane maintenance in these animals. Not only have these combined methodologies identified new facets of the impact of SCDs on the membrane, but they also have great potential to reveal many undiscovered regulators of phospholipid metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.