Evidence map›Paper›PMID 26528806›Full record

ArticleBiosensors & bioelectronics2016

A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection.

Peng Ning, Jiacheng Jiang, Longchun Li, Shuxin Wang, Haizhu Yu, Yan Feng, Manzhou Zhu, Buchang Zhang, Hang Yin, Qingxiang Guo and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Peng NingDepartment of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
Jiacheng JiangDepartment of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
Longchun LiDepartment of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
Shuxin WangDepartment of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
Haizhu YuDepartment of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
Yan FengDepartment of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
Manzhou ZhuDepartment of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
Buchang ZhangInstitute of Health Sciences, Anhui University, Hefei 230601, China.
Hang YinDepartment of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado at Boulder, Boulder, CO 80309, USA.
Qingxiang GuoDepartment of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Xiangming MengDepartment of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China. Electronic address: mengxm@ahu.edu.cn.

Funding

Exogenous Chemical Probes of TLR-Mediated NeuroinflammationR01GM101279 · NIGMS · UNIVERSITY OF COLORADO · PI YIN, HANG HUBERT · 2013 to 2017
$1.4M
Dissecting Cell Signaling Mediated by Protein-Protein Interactions in MembranesR01GM103843 · NIGMS · UNIVERSITY OF COLORADO · PI YIN, HANG HUBERT · 2013 to 2016
$1.3M
NIGMS NIH HHS R01 GM101279NIGMS NIH HHS R01GM101279NIGMS NIH HHS R01 GM103843
6 · The paper itself

Abstract

A mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MPVQ) for biological zinc ions detection was developed based on quinolone platform. Mito-MPVQ showed large red shifts (68 nm) and selective ratiometric signal upon Zn(2+) binding. The ratio of emission intensity (I488 nm/I420 nm) increases dramatically from 0.45 to 3.79 (ca. 8-fold). NMR titration and theoretical calculation confirmed the binding of Mito-MPVQ and Zn(2+). Mito-MPVQ also exhibited large two-photon absorption cross sections (150 GM) at nearly 720 nm and insensitivity to pH within the biologically relevant pH range. Cell imaging indicated that Mito-MPVQ could efficiently located in mitochondria and monitor mitochondrial Zn(2+) under two-photon excitation with low cytotoxicity.

Indexed as

AnimalsCHO CellsCricetulusFluorescent DyesMicroscopy, Fluorescence, MultiphotonMitochondriaMolecular ImagingMolecular Probe TechniquesReproducibility of ResultsSensitivity and SpecificityStaining and LabelingZincFluorescent DyesZincFluorescent probeMitochondria-targetedRatiometricTwo-photonZinc ions

Identifiers

PMID26528806
PMCPMC4673014

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.