Evidence map›Paper›PMID 26528633›Full record

ArticleCurrent protocols in immunology2015

Isolation and Functional Analysis of Human Neutrophils.

Douglas B Kuhns, Debra A Long Priel, Jessica Chu, Kol A Zarember

Abstract read
In one paragraph

Article in Current protocols in immunology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.

0numbers the graph read from it
0cells of the map it votes in
84citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

84 citing papers in PubMed.

  1. Article
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  17. Article
  18. Article
  19. Article
  20. Targeting Neutrophil βBiomolecules · 2023
    Review

24 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Douglas B KuhnsNeutrophil Monitoring Lab, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland.
Debra A Long PrielNeutrophil Monitoring Lab, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland.
Jessica ChuLaboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Kol A ZaremberLaboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.

Funding

Laboratory Studies of Abnormal Host Defense and Immunoregulatory DiseasesZIAAI001151 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI ZAREMBER, KOL · 2011 to 2022
$2.4M
CCR NIH HHS HHSN261200800001CIntramural NIH HHS Z99 AI999999Intramural NIH HHS ZIA AI001151NCI NIH HHS HHSN261200800001E
6 · The paper itself

Abstract

This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described.

Indexed as

Centrifugation, Density GradientCytochromes cDextransFicollFlow CytometryHumansHydrogen PeroxideNeutrophilsPhagocytosisPovidoneRespiratory BurstSilicon DioxideStaphylococcus aureusSuperoxide DismutaseCytochromes cDextransFicollHydrogen PeroxidePercollPovidoneSilicon DioxideSuperoxide Dismutaseinnate immunityleukocyteneutrophilNOX2phagocyte

Identifiers

PMID26528633
PMCPMC4668800

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.