ArticleNucleic acids research2016
The role of topoisomerase I in suppressing genome instability associated with a highly transcribed guanine-rich sequence is not restricted to preventing RNA:DNA hybrid accumulation.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 51 citations in OpenAlex.
- When R-Loops Go Awry: Genome Instability and Neurological Diseases.The European journal of neuroscience · 2026Review
- Mutations and Recombination at G4 DNA-Forming Sequences Exacerbated by CPT-Resistant Mutant Topoisomerase 1 Is Dependent on SUMOylation.International journal of molecular sciences · 2025Article
- G-quadruplexes in an SVA retrotransposon cause aberrant TAF1 gene expression in X-linked dystonia parkinsonism.Nucleic acids research · 2024Article
- DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in Arabidopsis.Nature communications · 2023Article
- Yeast transcription factor Msn2 binds to G4 DNA.Nucleic acids research · 2023Article
- Article
- DNA topoisomerase 1 represses HIV-1 promoter activity through its interaction with a guanine quadruplex present in the LTR sequence.Retrovirology · 2023Review
- G4-interacting proteins endangering genomic stability at G4 DNA-forming sites.Biochemical Society transactions · 2023Article
- Ligands stimulating antitumour immunity as the next G-quadruplex challenge.Molecular cancer · 2022Review
- Cleavage-defective Topoisomerase I mutants sharply increase G-quadruplex-associated genomic instability.Microbial cell (Graz, Austria) · 2022Article
- Article
- Differential responses of neurons, astrocytes, and microglia to G-quadruplex stabilization.Aging · 2021Article
- RTEL1 Regulates G4/R-Loops to Avert Replication-Transcription Collisions.Cell reports · 2020Article
- G-quadruplex-R-loop interactions and the mechanism of anticancer G-quadruplex binders.Nucleic acids research · 2020Review
- Article
- C1orf109L binding DHX9 promotes DNA damage depended on the R-loop accumulation and enhances camptothecin chemosensitivity.Cell proliferation · 2020Article
- The Functional Consequences of Eukaryotic Topoisomerase 1 Interaction with G-Quadruplex DNA.Genes · 2020Review
- RNase H activities counteract a toxic effect of Polymerase η in cells replicating with depleted dNTP pools.Nucleic acids research · 2019Article
- DNA damage and genome instability by G-quadruplex ligands are mediated by R loops in human cancer cells.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The Interplay between G-quadruplex and Transcription.Current medicinal chemistry · 2019Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Highly transcribed guanine-run containing sequences, in Saccharomyces cerevisiae, become unstable when topoisomerase I (Top1) is disrupted. Topological changes, such as the formation of extended RNA:DNA hybrids or R-loops or non-canonical DNA structures including G-quadruplexes has been proposed as the major underlying cause of the transcription-linked genome instability. Here, we report that R-loop accumulation at a guanine-rich sequence, which is capable of assembling into the four-stranded G4 DNA structure, is dependent on the level and the orientation of transcription. In the absence of Top1 or RNase Hs, R-loops accumulated to substantially higher extent when guanine-runs were located on the non-transcribed strand. This coincides with the orientation where higher genome instability was observed. However, we further report that there are significant differences between the disruption of RNase Hs and Top1 in regards to the orientation-specific elevation in genome instability at the guanine-rich sequence. Additionally, genome instability in Top1-deficient yeasts is not completely suppressed by removal of negative supercoils and further aggravated by expression of mutant Top1. Together, our data provide a strong support for a function of Top1 in suppressing genome instability at the guanine-run containing sequence that goes beyond preventing the transcription-associated RNA:DNA hybrid formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.