ArticleThe Journal of biological chemistry2015
The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition: INSIGHTS INTO SENSING MECHANISMS FOR PLASMA MEMBRANE LIPID ASYMMETRY.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Landscape of essential growth and fluconazole-resistance genes in the human fungal pathogen Cryptococcus neoformans.PLoS biology · 2025Article
- Alkaliphilic/Alkali-Tolerant Fungi: Molecular, Biochemical, and Biotechnological Aspects.Journal of fungi (Basel, Switzerland) · 2023Review
- Article
- Conserved and Divergent Features of pH Sensing in Major Fungal Pathogens.Current clinical microbiology reports · 2023Review
- Extracellular pH and high concentration of potassium regulate the primary necrosis in the yeast Saccharomyces cerevisiae.Archives of microbiology · 2021Article
- The Rim101 pathway mediates adaptation to external alkalization and altered lipid asymmetry: hypothesis describing the detection of distinct stresses by the Rim21 sensor protein.Current genetics · 2021Review
- The enigma of environmental pH sensing in plants.Nature plants · 2021Review
- Article
- N-glycosylation of Rim21 at an Unconventional Site Fine-tunes Its Behavior in the Plasma Membrane.Cell structure and function · 2020Article
- Identifying a novel connection between the fungal plasma membrane and pH-sensing.Molecular microbiology · 2018Article
- Characterization of additional components of the environmental pH-sensing complex in the pathogenic fungusThe Journal of biological chemistry · 2018Article
- Lipotoxicty in yeast: a focus on plasma membrane signalling and membrane contact sites.FEMS yeast research · 2018Review
- Diacylglycerol triggers Rim101 pathway-dependent necrosis in yeast: a model for lipotoxicity.Cell death and differentiation · 2018Article
- How alkalinization drives fungal pathogenicity.PLoS pathogens · 2017Review
- Stress Adaptation.Microbiology spectrum · 2017Review
- Mutational analysis of the Aspergillus ambient pH receptor PalH underscores its potential as a target for antifungal compounds.Molecular microbiology · 2016Article
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Authors and funding
3 authors.
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Abstract
Yeast responds to alterations in plasma membrane lipid asymmetry and external alkalization via the sensor protein Rim21 in the Rim101 pathway. However, the sensing mechanism used by Rim21 remains unclear. Here, we found that the C-terminal cytosolic domain of Rim21 (Rim21C) fused with GFP was associated with the plasma membrane under normal conditions but dissociated upon alterations in lipid asymmetry or external alkalization. This indicates that Rim21C contains a sensor motif. Rim21C contains multiple clusters of charged residues. Among them, three consecutive Glu residues (EEE motif) were essential for Rim21 function and dissociation of Rim21C from the plasma membrane in response to changes in lipid asymmetry. In contrast, positively charged residues adjacent to the EEE motif were required for Rim21C to associate with the membrane. We therefore propose an "antenna hypothesis," in which Rim21C moves to or from the plasma membrane and functions as the sensing mechanism of Rim21.
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