ArticleScientific reports2015
Genome-wide identification and characterization of reference genes with different transcript abundances for Streptomyces coelicolor.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 22 citations in OpenAlex.
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- Construction of Streptomyces coelicolor A3(2) mutants that exclusively produce NA4/NA6 intermediates of agarose metabolism through mutation induction.Scientific reports · 2023Article
- Complete genomes and comparative analyses of Streptomyces phages that influence secondary metabolism and sporulation.Scientific reports · 2023Article
- Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins EBioresources and bioprocessing · 2022Article
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- Molecular mechanism of GylR-mediated regulation of glycerol metabolism inFrontiers in microbiology · 2022Article
- Enhanced production of clavulanic acid by improving glycerol utilization using reporter-guided mutagenesis of an industrial Streptomyces clavuligerus strain.Journal of industrial microbiology & biotechnology · 2021Article
- Mining and fine-tuning sugar uptake system for titer improvement of milbemycins inSynthetic and systems biotechnology · 2020Article
- Article
- Harnessing the intracellular triacylglycerols for titer improvement of polyketides in Streptomyces.Nature biotechnology · 2020Article
- Cytosolic copper is a major modulator of germination, development and secondary metabolism in Streptomyces coelicolor.Scientific reports · 2019Article
- Genome-Wide Identification and Evaluation of New Reference Genes for Gene Expression Analysis Under Temperature and Salinity Stresses inFrontiers in genetics · 2019Article
- sRNA scr5239 Involved in Feedback Loop Regulation ofFrontiers in microbiology · 2019Article
- Recent advances in understandingF1000Research · 2016Review
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lack of reliable reference genes (RGs) in the genus Streptomyces hampers effort to obtain the precise data of transcript levels. To address this issue, we aimed to identify reliable RGs in the model organism Streptomyces coelicolor. A pool of potential RGs containing 1,471 genes was first identified by determining the intersection of genes with stable transcript levels from four time-series transcriptome microarray datasets of S. coelicolor M145 cultivated in different conditions. Then, following a strict rational selection scheme including homology analysis, disturbance analysis, function analysis and transcript abundance analysis, 13 candidates were selected from the 1,471 genes. Based on real-time quantitative reverse transcription PCR assays, SCO0710, SCO6185, SCO1544, SCO3183 and SCO4758 were identified as the top five genes with the most stable transcript levels among the 13 candidates. Further analyses showed these five genes also maintained stable transcript levels in different S. coelicolor strains, as well as in Streptomyces avermitilis MA-4680 and Streptomyces clavuligerus NRRL 3585, suggesting they could fulfill the requirements of accurate data normalization in streptomycetes. Moreover, the systematic strategy employed in this work could be used for reference in other microorganism to select reliable RGs.
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