ArticleBiomedical optics express2014
3D high-density localization microscopy using hybrid astigmatic/ biplane imaging and sparse image reconstruction.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Cellular optical imaging techniques: a dynamic advancing frontier.Science China. Life sciences · 2025Review
- DrSTAR: Tracking real-time nanometer axial changes.Biophysical journal · 2023Article
- Recent progress on single-molecule localization microscopy.Biophysics reports · 2021Article
- Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited].Optics express · 2018Review
- Localization-based super-resolution imaging meets high-content screening.Nature methods · 2017Article
- Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking.Chemical reviews · 2017Review
- Reconstruction of super-resolution STORM images using compressed sensing based on low-resolution raw images and interpolation.Biomedical optics express · 2017Article
- Generalized recovery algorithm for 3D super-resolution microscopy using rotating point spread functions.Scientific reports · 2016Article
- Correlated confocal and super-resolution imaging by VividSTORM.Nature protocols · 2016Article
- 3D multifocus astigmatism and compressed sensing (3D MACS) based superresolution reconstruction.Biomedical optics express · 2015Article
- Cell Cycle Machinery in Bacillus subtilis.Sub-cellular biochemistryReview
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Authors and funding
6 authors.
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Abstract
Localization microscopy achieves nanoscale spatial resolution by iterative localization of sparsely activated molecules, which generally leads to a long acquisition time. By implementing advanced algorithms to treat overlapping point spread functions (PSFs), imaging of densely activated molecules can improve the limited temporal resolution, as has been well demonstrated in two-dimensional imaging. However, three-dimensional (3D) localization of high-density data remains challenging since PSFs are far more similar along the axial dimension than the lateral dimensions. Here, we present a new, high-density 3D imaging system and algorithm. The hybrid system is implemented by combining astigmatic and biplane imaging. The proposed 3D reconstruction algorithm is extended from our state-of-the art 2D high-density localization algorithm. Using mutual coherence analysis of model PSFs, we validated that the hybrid system is more suitable than astigmatic or biplane imaging alone for 3D localization of high-density data. The efficacy of the proposed method was confirmed via simulation and real data of microtubules. Furthermore, we also successfully demonstrated fluorescent-protein-based live cell 3D localization microscopy with a temporal resolution of just 3 seconds, capturing fast dynamics of the endoplasmic recticulum.
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