ArticleScientific reports2015
Proteomic profiling of host-biofilm interactions in an oral infection model resembling the periodontal pocket.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 44 citations in OpenAlex.
- ZrOJournal of functional biomaterials · 2026Article
- The Subgingival Host-Microbial Landscape in Mothers With Periodontitis and Their At-Risk Offspring.Journal of clinical periodontology · 2026Article
- Advances in oral disease models: a mini-review of developments from 2015 to 2025.Frontiers in dental medicine · 2026Review
- Immunomodulatory effects of eubiotic and dysbiotic multi-species biofilms on oral keratinocytes.BMC oral health · 2025Article
- Saliva Versus All-Site Microbiome and Proteome Mapping in Periodontitis.Journal of clinical periodontology · 2025Article
- Microbiome composition and metabolic pathways in shallow and deep periodontal pockets.Scientific reports · 2025Article
- An in vitro model demonstrating homeostatic interactions between reconstructed human gingiva and a saliva-derived multispecies biofilm.Microbiome · 2025Article
- Advances in modeling periodontal host-microbe interactions: insights from organotypic and organ-on-chip systems.Lab on a chip · 2025Review
- Biofabrication Strategies for Oral Soft Tissue Regeneration.Advanced healthcare materials · 2024Review
- Microfluidic organ-on-chip systems for periodontal research: advances and future directions.Frontiers in bioengineering and biotechnology · 2024Review
- Microphysiological Modeling of Gingival Tissues and Host-Material Interactions Using Gingiva-on-Chip.Advanced healthcare materials · 2023Article
- Potential Clinical Application of Organs-on-a-Chip in Periodontal Diseases: A Systematic Review of In Vitro Studies.Dentistry journal · 2023Review
- Proteomics analysis of urine and catheter-associated biofilms in spinal cord injury patients.American journal of clinical and experimental urology · 2023Article
- Oral microbiome interactions with gingival gene expression patterns for apoptosis, autophagy and hypoxia pathways in progressing periodontitis.Immunology · 2021Article
- Three-DimensionalTissue engineering. Part B, Reviews · 2020Review
- A review of co-culture models to study the oral microenvironment and disease.Journal of oral microbiology · 2020Review
- A salivary metabolite signature that reflects gingival host-microbe interactions: instability predicts gingivitis susceptibility.Scientific reports · 2020Article
- Article
- Host-microbe interactions: Profiles in the transcriptome, the proteome, and the metabolome.Periodontology 2000 · 2020Review
- Virulence and Pathogenicity Properties ofPathogens (Basel, Switzerland) · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Periodontal infections cause inflammatory destruction of the tooth supporting tissues. We recently developed a dynamic, in vitro periodontal organotypic tissue model in a perfusion bioreactor system, in co-culture with an 11-species subgingival biofilm, which may recapitulate early events during the establishment of periodontal infections. This study aimed to characterize the global proteome regulations in this host-biofilm interaction model. Semi-quantitative shotgun proteomics were applied for protein identification and quantification in the co-culture supernatants (human and bacterial) and the biofilm lysates (bacterial). A total of 896 and 3363 proteins were identified as secreted in the supernatant and expressed in the biofilm lysate, respectively. Enriched gene ontology analysis revealed that the regulated secreted human tissue proteins were related to processes of cytoskeletal rearrangement, stress responses, apoptosis, and antigen presentation, all of which are commensurate with deregulated host responses. Most secreted bacterial biofilm proteins derived from their cytoplasmic domain. In the presence of the tissue, the levels of Fusobacterium nucleatum, Actinomyces oris and Campylobacter rectus proteins were significantly regulated. The functions of the up-regulated intracellular (biofilm lysate) proteins were associated with cytokinesis. In conclusion, the proteomic overview of regulated pathways in this host-biofilm interaction model provides insights to the early events of periodontal pathogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.