ArticleParticle and fibre toxicology2015
Proteomic analysis of protein carbonylation: a useful tool to unravel nanoparticle toxicity mechanisms.
Article in Particle and fibre toxicology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Toxic Effects of Copper and Zinc Oxide Nanoparticles on Brain Tissue Antioxidant Defense of Male Swiss Albino Mice.Biological trace element research · 2026Article
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- Proteomic insights to decipher nanoparticle uptake, translocation, and intercellular mechanisms in plants.Environmental science and pollution research international · 2024Review
- Differential impact of plant-based selenium nanoparticles on physio-biochemical properties, antioxidant defense system and protein regulation in fruits of huanglongbing-infected 'Kinnow' mandarin plants.Frontiers in plant science · 2024Article
- The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design.Nanomaterials (Basel, Switzerland) · 2023Review
- Protein carbonylation in food and nutrition: a concise update.Amino acids · 2022Article
- Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiONanomaterials (Basel, Switzerland) · 2021Article
- Refractive Index Changes of Cells and Cellular Compartments Upon Paraformaldehyde Fixation Acquired by Tomographic Phase Microscopy.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
- Anticancer Properties of Platinum Nanoparticles and Retinoic Acid: Combination Therapy for the Treatment of Human Neuroblastoma Cancer.International journal of molecular sciences · 2020Article
- Carbon dots for effective photodynamic inactivation of virus.RSC advances · 2020Article
- Assessment of DNA Methylation and Oxidative Changes in the Heart and Brain of Rats Receiving a High-Fat Diet Supplemented with Various Forms of Chromium.Animals : an open access journal from MDPI · 2020Article
- Melatonin Enhances Palladium-Nanoparticle-Induced Cytotoxicity and Apoptosis in Human Lung Epithelial Adenocarcinoma Cells A549 and H1229.Antioxidants (Basel, Switzerland) · 2020Article
- Predicting dissolution and transformation of inhaled nanoparticles in the lung using abiotic flow cells: The case of barium sulfate.Scientific reports · 2020Article
- Anisotropic Platinum Nanoparticle-Induced Cytotoxicity, Apoptosis, Inflammatory Response, and Transcriptomic and Molecular Pathways in Human Acute Monocytic Leukemia Cells.International journal of molecular sciences · 2020Article
- An in-depth multi-omics analysis in RLE-6TN rat alveolar epithelial cells allows for nanomaterial categorization.Particle and fibre toxicology · 2019Article
- Biological monitoring of workers exposed to engineered nanomaterials.Toxicology letters · 2018Review
- Mass spectrometry-based proteomics for system-level characterization of biological responses to engineered nanomaterials.Analytical and bioanalytical chemistry · 2018Review
- Carcinogenicity of multi-walled carbon nanotubes: challenging issue on hazard assessment.Journal of occupational health · 2018Article
- Systems Biology to Support Nanomaterial Grouping.Advances in experimental medicine and biology · 2017Review
- Comparative oxidative stress elicited by nanosilver in stableToxicology research · 2016Article
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14 authors.
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Abstract
backgroundOxidative stress, a commonly used paradigm to explain nanoparticle (NP)-induced toxicity, results from an imbalance between reactive oxygen species (ROS) generation and detoxification. As one consequence, protein carbonyl levels may become enhanced. Thus, the qualitative and quantitative description of protein carbonylation may be used to characterize how biological systems respond to oxidative stress induced by NPs.
methodsWe investigated a representative panel of 24 NPs including functionalized amorphous silica (6), zirconium dioxide (4), silver (4), titanium dioxide (3), zinc oxide (2), multiwalled carbon nanotubes (3), barium sulfate and boehmite. Surface reactivities of all NPs were studied in a cell-free system by electron spin resonance (ESR). NRK-52E cells were treated with all NPs, analyzed for viability (WST-1 assay) and intracellular ROS production (DCFDA assay). Carbonylated proteins were assessed by 1D and/or 2D immunoblotting and identified by matrix assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF/TOF). In parallel, tissue homogenates from rat lungs intratracheally instilled with silver NPs were studied.
resultsEleven NPs induced elevated levels of carbonylated proteins. This was in good agreement with the surface reactivity of the NPs as obtained by ESR and the reduction in cell viability as assessed by WST-1 assay. By contrast, results obtained by DCFDA assay were deviating. Each NP induced an individual pattern of protein carbonyls on 2D immunoblots. Affected proteins comprised cytoskeletal components, proteins being involved in stress response, or cytoplasmic enzymes of central metabolic pathways such as glycolysis and gluconeogenesis. Furthermore, induction of carbonyls upon silver NP treatment was also verified in rat lung tissue homogenates.
conclusionsAnalysis of protein carbonylation is a versatile and sensitive method to describe NP-induced oxidative stress and, therefore, can be used to identify NPs of concern. Furthermore, detailed information about compromised proteins may aid in classifying NPs according to their mode of action.
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