ArticleJournal of synchrotron radiation2015
In situ X-ray data collection and structure phasing of protein crystals at Structural Biology Center 19-ID.
Article in Journal of synchrotron radiation, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- In situ crystal data-collection and ligand-screening system at SPring-8.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Novel combined crystallization plate for high-throughput crystal screening and in situ data collection at a crystallography beamline.Acta crystallographica. Section F, Structural biology communications · 2021Article
- The low-cost Shifter microscope stage transforms the speed and robustness of protein crystal harvesting.Acta crystallographica. Section D, Structural biology · 2021Article
- ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF.Journal of synchrotron radiation · 2018Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
A prototype of a 96-well plate scanner for in situ data collection has been developed at the Structural Biology Center (SBC) beamline 19-ID, located at the Advanced Photon Source, USA. The applicability of this instrument for protein crystal diffraction screening and data collection at ambient temperature has been demonstrated. Several different protein crystals, including selenium-labeled, were used for data collection and successful SAD phasing. Without the common procedure of crystal handling and subsequent cryo-cooling for data collection at T = 100 K, crystals in a crystallization buffer show remarkably low mosaicity (<0.1°) until deterioration by radiation damage occurs. Data presented here show that cryo-cooling can cause some unexpected structural changes. Based on the results of this study, the integration of the plate scanner into the 19-ID end-station with automated controls is being prepared. With improvement of hardware and software, in situ data collection will become available for the SBC user program including remote access.
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Registered trials
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