ArticleNature methods2016
Fixed single-cell transcriptomic characterization of human radial glial diversity.
Article in Nature methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 152 papers.
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Who cites it
152 citing papers in PubMed.
- RNA-aware tissue preservation workflows for high-quality spatial transcriptomics.bioRxiv : the preprint server for biology · 2026Article
- Genomics in containment: BSL-4-compatible workflows enable high-resolution genomic and transcriptomic analyses of Risk Group 4 viruses.iScience · 2026Article
- Generation, differentiation, and potential of three radial glial subtypes in human cortical organoids.BMB reports · 2026Review
- Independent control of neurogenesis and dorsoventral patterning by NKX2-2.Genes & development · 2026Article
- Bridging unpaired single-cell multimodal data for integrative analyses with SuperMap.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Oriented cell divisions induce basal progenitors and regulate neural expansion across tissues and species.Science advances · 2026Article
- Application of single-cell RNA sequencing in preeclampsia.Frontiers in global women's health · 2026Review
- The recent advancements of single-cell RNA sequencing in pre-eclampsia.Frontiers in medicine · 2026Review
- POU3F2 regulates canonical Wnt signalling via SOX13 and ADNP to expand the neural progenitor population.Brain : a journal of neurology · 2025Article
- DNA replication fork speed acts as a pacer in cortical neurogenesis.Nature communications · 2025Article
- Thymosin beta 4 as an Alzheimer disease intervention target identified using human brain organoids.Stem cell reports · 2025Article
- Co-profiling of in situ RNA-protein interactions and transcriptome in single cells and tissues.Nature methods · 2025Article
- scGT:integration algorithm for single-cell RNA-seq and ATAC-seq based on graph transformer.Bioinformatics (Oxford, England) · 2025Article
- snCED-seq: high-fidelity cryogenic enzymatic dissociation of nuclei for single-nucleus RNA-seq of FFPE tissues.Nature communications · 2025Article
- A dyad of human-specificScience advances · 2025Article
- Review
- Viral reprogramming of glial metabolism as a driver of neuroinflammation.Frontiers in immunology · 2025Review
- Proliferating Microglia Exhibit Unique Transcriptional and Functional Alterations in Alzheimer's Disease.ASN neuro · 2025Article
- Microdissection and Single-Cell Suspension of Neocortical Layers From Ferret Brain for Single-Cell Assays.Bio-protocol · 2024Article
- Antiviral immunity within neural stem cells distinguishes Enterovirus-D68 strain differences in forebrain organoids.Journal of neuroinflammation · 2024Article
92 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1% of the midgestation cortex and classified them as ventricular zone-enriched RG (vRG) that express ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that express HOPX. Our study identified vRG and oRG markers and molecular profiles, an essential step for understanding human neocortical progenitor development. FRISCR allows targeted single-cell profiling of any tissues that lack live-cell markers.
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Registered trials
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