Evidence map›Paper›PMID 26523491›Full record

ArticleEndocrinology2016

Functional Reconstitution of the Insulin-Secreting Porosome Complex in Live Cells.

Akshata R Naik, Sanjana P Kulkarni, Kenneth T Lewis, Douglas J Taatjes, Bhanu P Jena

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Akshata R NaikDepartment of Physiology (A.R.N., S.P.K., K.T.L., B.P.J.), Wayne State University School of Medicine, Detroit, Michigan 48201; and Department of Pathology and Laboratory Medicine (D.J.T.), Microscopy Imaging Center, University of Vermont College of Medicine, Burlington, Vermont 05405.
Sanjana P KulkarniDepartment of Physiology (A.R.N., S.P.K., K.T.L., B.P.J.), Wayne State University School of Medicine, Detroit, Michigan 48201; and Department of Pathology and Laboratory Medicine (D.J.T.), Microscopy Imaging Center, University of Vermont College of Medicine, Burlington, Vermont 05405.
Kenneth T LewisDepartment of Physiology (A.R.N., S.P.K., K.T.L., B.P.J.), Wayne State University School of Medicine, Detroit, Michigan 48201; and Department of Pathology and Laboratory Medicine (D.J.T.), Microscopy Imaging Center, University of Vermont College of Medicine, Burlington, Vermont 05405.
Douglas J TaatjesDepartment of Physiology (A.R.N., S.P.K., K.T.L., B.P.J.), Wayne State University School of Medicine, Detroit, Michigan 48201; and Department of Pathology and Laboratory Medicine (D.J.T.), Microscopy Imaging Center, University of Vermont College of Medicine, Burlington, Vermont 05405.
Bhanu P JenaDepartment of Physiology (A.R.N., S.P.K., K.T.L., B.P.J.), Wayne State University School of Medicine, Detroit, Michigan 48201; and Department of Pathology and Laboratory Medicine (D.J.T.), Microscopy Imaging Center, University of Vermont College of Medicine, Burlington, Vermont 05405.

Funding

UNDERSTANDING MEMBRANE FUSION A BIOENGINEERING APPROACHR01EB000303 · NIBIB · WAYNE STATE UNIVERSITY · PI JENA, BHANU P · 2002 to 2004
$1.2M
EXOCYTOSIS: USING NANOSURGERY AND BIOENGINEERINGR01DK056212 · NIDDK · YALE UNIVERSITY · PI JENA, BHANU P · 1999 to 2003
$1.1M
NIBIB NIH HHS R01 EB000303NIDDK NIH HHS DK56212NIDDK NIH HHS R01 DK056212
6 · The paper itself

Abstract

Supramolecular cup-shaped lipoprotein structures called porosomes embedded in the cell plasma membrane mediate fractional release of intravesicular contents from cells during secretion. The presence of porosomes, have been documented in many cell types including neurons, acinar cells of the exocrine pancreas, GH-secreting cells of the pituitary, and insulin-secreting pancreatic β-cells. Functional reconstitution of porosomes into artificial lipid membranes, have also been accomplished. Earlier studies on mouse insulin-secreting Min6 cells report 100-nm porosome complexes composed of nearly 30 proteins. In the current study, porosomes have been functionally reconstituted for the first time in live cells. Isolated Min6 porosomes reconstituted into live Min6 cells demonstrate augmented levels of porosome proteins and a consequent increase in the potency and efficacy of glucose-stimulated insulin release. Elevated glucose-stimulated insulin secretion 48 hours after reconstitution, reflects on the remarkable stability and viability of reconstituted porosomes, documenting the functional reconstitution of native porosomes in live cells. These results, establish a new paradigm in porosome-mediated insulin secretion in β-cells.

Indexed as

AnimalsCell Line, TumorGTP-Binding Protein alpha Subunits, Gi-GoHyperglycemiaInsulinInsulin-Secreting CellsInsulin SecretionMembrane MicrodomainsMiceMicroscopy, Atomic ForceMicroscopy, Electron, TransmissionMicroscopy, FluorescencePotassium Channels, Tandem Pore DomainProtein StabilityProtein TransportScattering, Small AngleGnai3 protein, mouseGTP-Binding Protein alpha Subunits, Gi-GoInsulinpotassium channel protein TREK-1Potassium Channels, Tandem Pore DomainSnap25 protein, mouseStx1a protein, mouseSynaptosomal-Associated Protein 25Syntaxin 1

Identifiers

PMID26523491
PMCPMC4701877

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.