ArticleScientific reports2015
A novel electroporation system for efficient molecular delivery into Chlamydomonas reinhardtii with a 3-dimensional microelectrode.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Leveraging electric current stimulation and chemical induction to enhance MSC differentiation into cardiomyocytes.Regenerative medicine · 2025Article
- A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall.Scientific reports · 2019Article
- Rapid and high efficiency transformation ofBioscience reports · 2019Article
- Microfluidic techniques for enhancing biofuel and biorefinery industry based on microalgae.Biotechnology for biofuels · 2019Article
- Biomass from microalgae: the potential of domestication towards sustainable biofactories.Microbial cell factories · 2018Review
- A comparison of inverted and upright laser-activated titanium nitride micropyramids for intracellular delivery.Scientific reports · 2018Article
- Nanobodies: Chemical Functionalization Strategies and Intracellular Applications.Angewandte Chemie (International ed. in English) · 2018Review
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Authors and funding
3 authors.
Funding
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Abstract
Electroporation is one of the most widely used transfection methods because of its high efficiency and convenience among the various transfection methods. Previous micro-electroporation systems have some drawbacks such as limitations in height and design, time-consuming and an expensive fabrication process due to technical constraints. This study fabricates a three dimensional microelectrode using the 3D printing technique. The interdigitated microstructure consisting of poly lactic acid was injected by a 3D printer and coated with silver and aluminum with a series of dip-coatings. With the same strength of electric field (V cm(-1)), a higher efficiency for molecular delivery and a higher cellular viability are achieved with the microelectrode than with a standard cuvette. In addition, this study investigates chemicophysical changes such as Joule heating and dissolved metal during electroporation and showed the micro-electroporation system had less chemicophysical changes. It was concluded that the proposed micro-electroporation system will contribute to genetic engineering as a promising delivery tool, and this combination of 3D printing and electroporation has many potential applications for diverse designs or systems.
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