ArticleAnnals of laboratory medicine2016
Basophil markers for identification and activation in the indirect basophil activation test by flow cytometry for diagnosis of autoimmune urticaria.
Article in Annals of laboratory medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 44 citations in OpenAlex.
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- Diagnostic Use of CCR3, CD63, CD203c and FcεRIα on Blood Leukocytes of Allergic Asthma and Combined Allergic Rhinitis and Asthma Syndrome.Journal of cellular and molecular medicine · 2025Article
- Eosinophils, basophils and myeloid-derived suppressor cells in chronic Loa loa infection and its treatment in an endemic setting.PLoS neglected tropical diseases · 2024Article
- Towards standardizing basophil identification by flow cytometry.Frontiers in allergy · 2023Article
- Altered Expression of Substance P and NK1R in CCR3Allergy, asthma & immunology research · 2022Article
- Increased expressions of CD123, CD63, CD203c, and Fc epsilon receptor I on blood leukocytes of allergic asthma.Frontiers in molecular biosciences · 2022Article
- Profiling Glioblastoma Cases with an Expression of DCX, OLIG2 and NES.International journal of molecular sciences · 2021Article
- Validation of inducible basophil biomarkers: Time, temperature and transportation.Cytometry. Part B, Clinical cytometry · 2021Article
- Behind the scenes with basophils: an emerging therapeutic target.Immunotherapy advances · 2021Review
- The Absolute Basophil Count.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Neutrophil oxidative burst as a diagnostic indicator of IgG-mediated anaphylaxis.Blood research · 2018Article
- Basophil Activation Test for Chronic Urticaria.Annals of laboratory medicine · 2016Article
- Reply to the Letter to the Editor by Dr. Chirumbolo.Annals of laboratory medicine · 2016Article
- Commentary: The Expression of CD123 Can Decrease with Basophil Activation: Implications for the Gating Strategy of the Basophil Activation Test.Frontiers in immunology · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe indirect basophil activation test using flow cytometry is a promising tool for autoimmune urticaria diagnosis. We aimed to identify better donor basophils (from atopic vs. non-atopic donors and interleukin-3 primed vs. unprimed basophils) and improve basophil identification and activation markers (eotaxin CC chemokine receptor-3 [CCR3] vs. CD123 and CD63 vs. CD203c).
methodsDonor basophils were obtained from non-atopic and atopic group O donors. Positive control sera were artificially prepared to simulate autoimmune urticaria patients' sera. Patient sera were obtained from nine children with chronic urticaria. Assay sensitivity was compared among each variation by using positive control sera (n=21), applying cutoff values defined from negative control sera (n=20).
resultsFor basophil identification, a combination of CCR3 and CD123 markers revealed a higher correlation with automated complete blood count (r=0.530) compared with that observed using CD123 (r=0.498) or CCR3 alone (r=0.195). Three activation markers on the atopic donor basophils attained 100% assay sensitivity: CD203c on unprimed basophils, CD63+CD203+ or CD63 alone on primed basophils; however, these markers on the non-atopic donor basophils attained lower assay sensitivity.
conclusionsFor basophil identification markers, a combination of CD123 and CCR3 is recommended, while CD123 alone may be used as an alternative. Donor basophils should be obtained from an atopic donor. For basophil activation markers, either CD203c alone on unprimed basophils or CD203c and CD63 on primed basophils are recommended, while CD63 alone on primed basophils may be used as an alternative.
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