ArticleAnnals of laboratory medicine2016
Rapid detection of Pseudomonas aeruginosa and Acinetobacter baumannii Harboring bla(VIM-2), bla(IMP-1) and bla(OXA-23) genes by using loop-mediated isothermal amplification methods.
Article in Annals of laboratory medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Development of Loop-Mediated Isothermal Amplification with a Lateral Flow Dipstick for Detection of theInternational journal of molecular sciences · 2026Article
- Carbapenem-ResistantMicroorganisms · 2025Review
- On-Site Dual Detection of AirborneMicroorganisms · 2025Article
- Isothermal Amplification and Hypersensitive Fluorescence Dual-Enhancement Nucleic Acid Lateral Flow Assay for Rapid Detection ofBiosensors · 2023Article
- A Loop-mediated Isothermal Amplification With a Nanoparticle-Based Lateral Flow Biosensor Assay to Detect Pseudomonas aeruginosa in Endophthalmitis.Translational vision science & technology · 2021Article
- Design of Rapid Detection System for Five Major Carbapenemase Families (Infection and drug resistance · 2021Article
- Characteristics of Isolates ofFrontiers in microbiology · 2021Article
- Development of Loop-Mediated Isothermal Amplification Rapid Diagnostic Assays for the Detection ofFrontiers in molecular biosciences · 2021Article
- Identification of Acinetobacter baumannii and its carbapenem-resistant gene blaScientific reports · 2019Article
- Rapid molecular detection of macrolide resistance.BMC infectious diseases · 2019Article
- Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms.Journal of applied microbiology · 2018Review
- Aptamers in the Therapeutics and Diagnostics Pipelines.Theranostics · 2018Review
- Article
- Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) Using Simultaneous Detection of mecA, nuc, and femB by Loop-Mediated Isothermal Amplification (LAMP).Current microbiology · 2017Article
- Rapid and simple identification of carbapenemase genes, blaWorld journal of microbiology & biotechnology · 2017Article
- Surveillance of Dihydropteroate Synthase Genes inFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCarbapenem-resistant Pseudomonas aeruginosa (CRPA) and Acinetobacter baumannii (CRAB) are the leading causes of nosocomial infections. A rapid and sensitive test to detect CRPA and CRAB is required for appropriate antibiotic treatment. We optimized a loop-mediated isothermal amplification (LAMP) assay to detect the presence of bla(VIM-2), bla(IMP-1), and bla(OXA-23), which are critical components for carbapenem resistance.
methodsTwo sets of primers, inner and outer primers, were manually designed as previously described. The LAMP buffer was optimized (at 2mM MgSO₄) by testing different concentrations of MgSO₄. The optimal reaction temperature and incubation time were determined by using a gradient thermocycler. Then, the optimized bla(VIM-2), bla(IMP-1), and bla(OXA-23) LAMP reactions were evaluated by using 120 P. aeruginosa and 99 A. baumannii clinical isolates.
resultsOnly one strain of the 100 CRPA isolates harbored bla(IMP-1), whereas none of them harbored bla(VIM-2). These results indicate that the acquisition of bla(VIM-2) or bla(IMP-1) may not play a major role in carbapenem resistance in Korea. Fifty two strains of the 75 CRAB isolates contained bla(OXA-23), but none contained bla(VIM-2) and bla(IMP-1) alleles.
conclusionsOur results demonstrate the usefulness of LAMP for the diagnosis of CRPA and CRAB.
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