ArticleProteins2016
Toward rational thermostabilization of Aspergillus oryzae cutinase: Insights into catalytic and structural stability.
Article in Proteins, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 58 citations in OpenAlex.
- Computational identification of plastic-degrading enzymes in ocean microbiomes.Scientific reports · 2025Article
- Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes inBioengineering (Basel, Switzerland) · 2025Review
- Perspectives on the microorganisms with the potentials of PET-degradation.Frontiers in microbiology · 2025Review
- Widespread adaptive evolution in angiosperm photosystems provides insight into the evolution of photosystem II repair.The Plant cell · 2024Article
- AsiteDesign: a Semirational Algorithm for an Automated Enzyme Design.The journal of physical chemistry. B · 2023Article
- Structure-function analysis of two closely related cutinases from Thermobifida cellulosilytica.Biotechnology and bioengineering · 2022Article
- A Review of Advanced Molecular Engineering Approaches to Enhance the Thermostability of Enzyme Breakers: From Prospective of Upstream Oil and Gas Industry.International journal of molecular sciences · 2022Review
- Residue-Specific Incorporation of the Non-Canonical Amino Acid Norleucine Improves Lipase Activity on Synthetic Polyesters.Frontiers in bioengineering and biotechnology · 2022Article
- Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.International journal of molecular sciences · 2021Review
- Microbial lipases and their industrial applications: a comprehensive review.Microbial cell factories · 2020Review
- Enzymatic Remediation of Polyethylene Terephthalate (PET)-Based Polymers for Effective Management of Plastic Wastes: An Overview.Frontiers in bioengineering and biotechnology · 2020Review
- Microbial Degradation and Valorization of Plastic Wastes.Frontiers in microbiology · 2020Review
- Activity and Thermostability of GH5 Endoglucanase Chimeras from Mesophilic and Thermophilic Parents.Applied and environmental microbiology · 2019Article
- Active Site Flexibility as a Hallmark for Efficient PET Degradation by I. sakaiensis PETase.Biophysical journal · 2018Article
- Review: Engineering of thermostable enzymes for industrial applications.APL bioengineering · 2018Review
- Identification of a hot-spot to enhanceRSC advances · 2018Article
- High-level expression and characterization of a novel cutinase fromBiotechnology for biofuels · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Cutinases are powerful hydrolases that can cleave ester bonds of polyesters such as poly(ethylene terephthalate) (PET), opening up new options for enzymatic routes for polymer recycling and surface modification reactions. Cutinase from Aspergillus oryzae (AoC) is promising owing to the presence of an extended groove near the catalytic triad which is important for the orientation of polymeric chains. However, the catalytic efficiency of AoC on rigid polymers like PET is limited by its low thermostability; as it is essential to work at or over the glass transition temperature (Tg) of PET, that is, 70 °C. Consequently, in this study we worked toward the thermostabilization of AoC. Use of Rosetta computational protein design software in conjunction with rational design led to a 6 °C improvement in the thermal unfolding temperature (Tm) and a 10-fold increase in the half-life of the enzyme activity at 60 °C. Surprisingly, thermostabilization did not improve the rate or temperature optimum of enzyme activity. Three notable findings are presented as steps toward designing more thermophilic cutinase: (a) surface salt bridge optimization produced enthalpic stabilization, (b) mutations to proline reduced the entropy loss upon folding, and (c) the lack of a correlative increase in the temperature optimum of catalytic activity with thermodynamic stability suggests that the active site is locally denatured at a temperature below the Tm of the global structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.