ArticleDiagnostic pathology2015
Protective role of silymarin in a mouse model of renal Ischemia-Reperfusion injury.
Article in Diagnostic pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 27 citations in OpenAlex.
- Influence of a diet with potential geroprotectors on blood biochemistry and aging-associated markers in domestic cats: a pilot study.Biogerontology · 2025Article
- Herbal antioxidants in dialysis patients: a review of potential mechanisms and medical implications.Renal failure · 2021Review
- A Review of the Role of Flavonoids in Peptic Ulcer (2010-2020).Molecules (Basel, Switzerland) · 2020Review
- The effect of adipose-derived mesenchymal stem cells on renal function and histopathology in a rat model of ischemia-reperfusion induced acute kidney injury.Iranian journal of basic medical sciences · 2020Article
- The protective effects of silymarin on ischemia-reperfusion injuries: A mechanistic review.Iranian journal of basic medical sciences · 2019Review
- Retraction Note: Protective role of silymarin in a mouse model of renal Ischemia-Reperfusion injury.Diagnostic pathology · 2018Article
- Intraperitoneal Administration of Silymarin Protects End Organs from Multivisceral Ischemia/Reperfusion Injury in a Rat Model.Brazilian journal of cardiovascular surgeryArticle
- A systematic review of silymarin and silibinin mechanisms for attenuating cerebral ischemia-reperfusion injuries.Avicenna journal of phytomedicineReview
Corrections and comments
- Retraction · 2018-09-12Concerns/Issues about Data · Concerns/Issues about Image · Error in Image · Error in Methods · Unreliable Results and/or Conclusions ·
- Retracted
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe investigated the mechanism of action of silymarin in a mouse model of renal ischemia-reperfusion injury (I/R) to ascertain its role in the treatment of I/R injury.
methodsTwenty-four C57BL/6 male mice were divided randomly into three groups: control (sham); ischemia-reperfusion (I/R); silymarin + ischemia-reperfusion (silymarin + I/R). In sham mice, an abdominal incision was made, followed by dissection of the bilateral renal pedicle, with no further cross-clamping of arteries. Silymarin + I/R mice were administered 100 mg/kg silymarin daily for 7 consecutive days before surgery, whereas I/R mice were administered (i.g.) 0.9 % saline + 0.1 % (v/v) ethanol daily for 7 consecutive days before surgery. Silymarin + I/R and I/R mice were subjected to renal ischemia to induce acute kidney injury after 45-min clamping of bilateral renal arteries. Serum levels of creatinine and blood urea nitrogen levels were measured. Periodic acid-Schiff (PAS) staining was undertaken to detect damaged renal tissue. Myeloperoxidase (MPO) activity and immunofluorescent detection of CD68 expression was undertaken for each group. Levels of inflammatory cytokines secreted by renal tissue were monitored by ELISA. Apoptosis was detected by TUNEL staining. Expression of cleaved-caspase-3, Bcl-2 and Bax was detected by western blotting.
resultsSerum creatinine and blood urea nitrogen levels were elevated in silymarin + I/R and I/R groups compared with sham mice (p < 0.05), whereas those in the I/R group were significantly higher than in the silymarin + I/R group (p < 0.05). Number of damaged renal tubule cells and apoptotic cells in sham and silymarin + I/R groups was significantly lower than in I/R mice. MPO activity and secretion of inflammatory cytokines in silymarin + I/R and I/R groups was reduced (p < 0.05), and CD68 expression in silymarin + I/R mice was lower than in I/R mice (p < 0.05). Expression of cleaved-caspase-3 and Bax in the I/R group was significantly higher than in sham mice, whereas Bcl-2 expression was lower than in silymarin + I/R mice (p < 0.05).
conclusionsSilymarin can inhibit renal I/R injury by inhibiting release of inflammatory factors and regulating apoptosis.
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