ArticleAnalytical and bioanalytical chemistry2016
Assessment of the real-time PCR and different digital PCR platforms for DNA quantification.
Article in Analytical and bioanalytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 97 citations in OpenAlex.
- Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events.GM crops & food · 2026Article
- One Health Perspective of Mycoplasmas: Proposals for Standardizing In Vitro PK/PD Models of Human Mycoplasma Species by Referencing Veterinary Experiences.Microorganisms · 2026Review
- Comparison of two digital PCR platforms for quantification of genetically modified soybean events.GM crops & food · 2025Article
- Digital PCR assays for quantifying trichothecene-producing Fusarium species, including Fusarium langsethiae, F. poae, and F. sporotrichioides, in oats.Analytical and bioanalytical chemistry · 2025Article
- Development and Validation of a High-Sensitivity Droplet Digital PCR Assay for Serum Hepatitis B Virus DNA Detection.Journal of viral hepatitis · 2025Article
- Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR.Foods (Basel, Switzerland) · 2024Article
- One-Year Monitoring of Prevalence and Diversity of Dairy Propionic Acid Bacteria in Raw Milk by Means of Culture-Dependent and Culture-Independent Methods.Foods (Basel, Switzerland) · 2024Article
- Developing centrifugal force real-time digital PCR for detecting extremely low DNA concentration.Scientific reports · 2024Article
- Quantification of low-level human cytomegalovirus and Epstein-Barr virus DNAemia by digital PCR.Journal of virological methods · 2024Article
- Improved Canker Processing and Viability Droplet Digital PCR Allow Detection ofMicroorganisms · 2024Article
- Long COVID: Molecular Mechanisms and Detection Techniques.International journal of molecular sciences · 2023Review
- Droplet Digital PCR (ddPCR) Does Not Enhance the Sensitivity of Detection of Cytomegalovirus (CMV) DNA in Newborn Dried Blood Spots Evaluated in the Context of Newborn Congenital CMV (cCMV) Screening.International journal of neonatal screening · 2023Article
- Long-term study on wastewater SARS-CoV-2 surveillance across United Arab Emirates.The Science of the total environment · 2023Article
- Vibrio-Sequins - dPCR-traceable DNA standards for quantitative genomics of Vibrio spp.BMC genomics · 2023Article
- Assessment of virus concentration methods for detecting SARS-CoV-2 IN wastewater.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements.Analytical and bioanalytical chemistry · 2022Review
- Detection and quantification ofFrontiers in cellular and infection microbiology · 2022Article
- The Viral Load of Human Cytomegalovirus Infection in Children following Hematopoietic Stem Cell Transplant by Chip Digital PCR.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2022Article
- Development of real-time PCR and droplet digital PCR based marker for the detection ofFrontiers in plant science · 2022Article
- A droplet digital PCR assay for detection and quantification ofFrontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Digital PCR (dPCR) is beginning to supersede real-time PCR (qPCR) for quantification of nucleic acids in many different applications. Several analytical properties of the two most commonly used dPCR platforms, namely the QX100 system (Bio-Rad) and the 12.765 array of the Biomark system (Fluidigm), have already been evaluated and compared with those of qPCR. However, to the best of our knowledge, direct comparison between the three of these platforms using the same DNA material has not been done, and the 37 K array on the Biomark system has also not been evaluated in terms of linearity, analytical sensitivity and limit of quantification. Here, a first assessment of qPCR, the QX100 system and both arrays of the Biomark system was performed with plasmid and genomic DNA from human cytomegalovirus. With use of PCR components that alter the efficiency of qPCR, each dPCR platform demonstrated consistent copy-number estimations, which indicates the high resilience of dPCR. Two approaches, one considering the total reaction volume and the other considering the effective reaction size, were used to assess linearity, analytical sensitivity and variability. When the total reaction volume was considered, the best performance was observed with qPCR, followed by the QX100 system and the Biomark system. In contrast, when the effective reaction size was considered, all three platforms showed almost equal limits of detection and variability. Although dPCR might not always be more appropriate than qPCR for quantification of low copy numbers, dPCR is a suitable method for robust and reproducible quantification of viral DNA, and a promising technology for the higher-order reference measurement method.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.