Evidence map›Paper›PMID 26521056›Full record

ArticleVeterinary research communications2015

Reduction of enterotoxin induced fluid accumulation in ileal loops of neonatal calves with anti-F5 fimbriae recombinant antibody.

Alfredo Sahagun-Ruiz, Leticia V Velazquez, Shoba Bhaskaran, Chris M Jay, E Morales-Salinas, Keerti Rathore, Gale G Wagner, Suryakant D Waghela

Abstract read
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In one paragraph

Article in Veterinary research communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Changes in Gut Microbiota by theFrontiers in cellular and infection microbiology · 2022
    Article
  3. Article
  4. Review
  5. Recombinant Antibodies in Veterinary Medicine: An Update.Frontiers in veterinary science · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Alfredo Sahagun-RuizDepartamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Copilco Coyoacán, CP, 04510, México D.F., México. sahagun@unam.mx.
Leticia V VelazquezDepartamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Copilco Coyoacán, CP, 04510, México D.F., México.
Shoba BhaskaranDepartment of Veterinary Pathobiology, Texas A&M University, College Station, TX, 77843-4467, USA.
Chris M JayDepartment of Veterinary Pathobiology, Texas A&M University, College Station, TX, 77843-4467, USA.
E Morales-SalinasDepartamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Copilco Coyoacán, CP, 04510, México D.F., México.
Keerti RathoreInstitute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX, 77843-2123, USA.
Gale G WagnerDepartment of Veterinary Pathobiology, Texas A&M University, College Station, TX, 77843-4467, USA.
Suryakant D WaghelaDepartment of Veterinary Pathobiology, Texas A&M University, College Station, TX, 77843-4467, USA.
Texas A&M University · USUniversidad Nacional Autónoma de México · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neonatal calf colibacillosis caused by enterotoxigenic Escherichia coli (ETEC) is an economically significant problem in most parts of the world. The most common ETEC found in calves express the F5 (K99) fimbriae, which are necessary for the attachment of the bacteria to the ganglioside receptors on enterocytes. It is known that prevention of ETEC F5(+) adhesion to its ganglioside receptors with specific antibodies protects calves from colibacillosis. Previously we have described the development and characterization of a mouse recombinant antibody fragment (moRAb) that prevents F5 fimbrial protein induced agglutination of horse red blood cells (HRBC), which exhibit the same gangloside receptor for F5 fimbriae. Here we demonstrate that this recombinant antibody fragment inhibits in vitro the attachment of ETEC F5(+) bacteria to HRBC as well as isolated calf enterocytes, and in vivo it decreases fluid accumulation in intestinal loops of calves. Thus, correct oral administration of this anti-F5 moRAb may serve as an immunoprophylactic for cost effective control of colibacillosis in calves.

Indexed as

AnimalsAnimals, NewbornAntibodies, BacterialBacterial AdhesionCattleCattle DiseasesEnterocytesEnterotoxinsErythrocytesEscherichia coliEscherichia coli InfectionsEscherichia coli ProteinsFimbriae, BacterialHorsesIleumMaleAntibodies, BacterialEnterotoxinsEscherichia coli ProteinsRecombinant ProteinsAdhesion: calf intestinal loopsBovine enterocytesEnterotoxigenic Escherichia coliFimbriae F5Recombinant antibodySingle-chain variable fragment

Identifiers

PMID26521056
OpenAlexW1923901568

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.