ArticlePlant molecular biology2016
L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis.
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 93 citations in OpenAlex.
- PCIS1 is an essential factor in mitochondrial RNA splicing and complex I biogenesis, with distinct effects in null and downregulated mutants.Plant cell reports · 2026Article
- Genome-wide characterization of soybean alcohol dehydrogenase (ADH) genes identifies GmADH13 as a positive regulator of the salt stress response.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- GhGLDH35A gene-mediated ROS homeostasis and stomatal movement via the ascorbic acid pathway confers alkaline stress tolerance.Journal of advanced research · 2026Article
- Disturbed RNA editing in MORF3-deficient Arabidopsis mitochondria leads to impaired assembly of complex I.Plant physiology · 2025Article
- The complex I subunit B22 contains a LYR domain that is crucial for an interaction with the mitochondrial acyl carrier protein SDAP1.The Plant journal : for cell and molecular biology · 2025Article
- C1-FDX is required for the assembly of mitochondrial complex I and subcomplexes of complex V in Arabidopsis.PLoS genetics · 2024Article
- The Arabidopsis Mitochondrial Nucleoid-Associated Protein WHIRLY2 Is Required for a Proper Response to Salt Stress.Plant & cell physiology · 2024Article
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Using cryo-EM to understand the assembly pathway of respiratory complex I.Acta crystallographica. Section D, Structural biology · 2024Article
- FTSH PROTEASE 3 facilitates Complex I degradation through a direct interaction with the Complex I subunit PSST.The Plant cell · 2023Article
- The biogenesis and regulation of the plant oxidative phosphorylation system.Plant physiology · 2023Review
- Identification of the GDP-L-Galactose Phosphorylase Gene as a Candidate for the Regulation of Ascorbic Acid Content in Fruits ofInternational journal of molecular sciences · 2023Article
- Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis.Plant physiology · 2023Article
- The Functions of Chloroplastic Ascorbate in Vascular Plants and Algae.International journal of molecular sciences · 2023Review
- Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway.Frontiers in plant science · 2023Review
- Metabolism and Regulation of Ascorbic Acid in Fruits.Plants (Basel, Switzerland) · 2022Review
- The perils of planning strategies to increase vitamin C content in plants: Beyond the hype.Frontiers in plant science · 2022Review
- Maturation and Assembly of Iron-Sulfur Cluster-Containing Subunits in the Mitochondrial Complex I From Plants.Frontiers in plant science · 2022Review
- Inactivation of mitochondrial complex I stimulates chloroplast ATPase in Physcomitrium patens.Plant physiology · 2021Article
- FTSH4 and OMA1 mitochondrial proteases reduce moderate heat stress-induced protein aggregation.Plant physiology · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-Galactono-1,4-lactone dehydrogenase (GLDH) catalyses the last enzymatic step of the ascorbate biosynthetic pathway in plants. GLDH is localised to mitochondria and several reports have shown that GLDH is associated with complex I of the respiratory chain. In a gldh knock-out mutant, complex I is not detectable, suggesting that GLDH is essential for complex I assembly or stability. GLDH has not been identified as a genuine complex I subunit, instead, it is present in a smaller, lowly abundant version of complex I called complex I*. In addition, GLDH activity has also been detected in smaller protein complexes within mitochondria membranes. Here, we investigated the role of GLDH during complex I assembly. We identified GLDH in complexes co-localising with some complex I assembly intermediates. Using a mutant that accumulates complex I assembly intermediates, we confirmed that GLDH is associated with the complex I assembly intermediates of 400 and 450 kDa. In addition, we detected accumulation of the 200 kDa complex I assembly intermediate in the gldh mutant. Taken together, our data suggest that GLDH is an assembly factor of the membrane arm of complex I. This function appears to be independent of the role of GLDH in ascorbate synthesis, as evidenced by the ascorbate-deficient mutant vtc2-1 accumulating wild-type levels of complex I. Therefore, we propose that GLDH is a dual-function protein that has a second, non-enzymatic function in complex I assembly as a plant-specific assembly factor. We propose an updated model for complex I assembly that includes complex I* as an assembly intermediate.
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