ArticlePlant molecular biology2016
New insight into the catalytic properties of rice sucrose synthase.
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- The Microbial Community Succession Drives Stage-Specific Carbon Metabolic Shifts DuringMicroorganisms · 2025Article
- Natural variation of WBR7 confers rice high yield and quality by modulating sucrose supply in sink organs.Plant biotechnology journal · 2024Article
- Multi-omics analyses reveal the mechanisms underlying the responses of Casuarina equisetifolia ssp. incana to seawater atomization and encroachment stress.BMC plant biology · 2024Article
- Whole-Genome Resequencing Identifies SNPs in Sucrose Synthase and Sugar Transporter Genes Associated with Sweetness in Coconut.Plants (Basel, Switzerland) · 2024Article
- Current perspectives on the regulatory mechanisms of sucrose accumulation in sugarcane.Heliyon · 2024Review
- Meta-QTL and haplo-pheno analysis reveal superior haplotype combinations associated with low grain chalkiness under high temperature in rice.Frontiers in plant science · 2023Article
- Molecular identification and expression analysis of five sucrose synthase genes inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Consuming Different Structural Parts of Bamboo Induce Gut Microbiome Changes in Captive Giant Pandas.Current microbiology · 2021Article
- Mutation of UDP-glucose binding motif residues lead to increased affinity for ADP-glucose in sugarcane sucrose phosphate synthase.Molecular biology reports · 2021Article
- Phosphoproteomic Profiling Reveals Early Salt-Responsive Mechanisms in Two Foxtail Millet Cultivars.Frontiers in plant science · 2021Article
- An Overview of Sucrose Synthases in Plants.Frontiers in plant science · 2019Review
- Thermo-responsive allele ofBreeding science · 2018Article
- Exploring natural genetic variation in tomato sucrose synthases on the basis of increased kinetic properties.PloS one · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sucrose synthase (SuS), which catalyzes the reversible conversion of sucrose and uridine diphosphate (UDP) into fructose and UDP-glucose, is a key enzyme in sucrose metabolism in higher plants. SuS belongs to family 4 of the glycosyltransferases (GT4) and contains an E-X7-E motif that is conserved in members of GT4 and two other GT families. To gain insight into the roles of this motif in rice sucrose synthase 3 (RSuS3), the two conserved glutamate residues (E678 and E686) in this motif and a phenylalanine residue (F680) that resides between the two glutamate residues were changed by site-directed mutagenesis. All mutant proteins maintained their tetrameric conformation. The mutants E686D and F680Y retained partial enzymatic activity and the mutants E678D, E678Q, F680S, and E686Q were inactive. Substrate binding assays indicated that UDP and fructose, respectively, were the leading substrates in the sucrose degradation and synthesis reactions of RSuS3. Mutations on E678, F680, and E686 affected the binding of fructose, but not of UDP. The results indicated that E678, F680, and E686 in the E-X7-E motif of RSuS3 are essential for the activity of the enzyme and the sequential binding of substrates. The sequential binding of the substrates implied that the reaction catalyzed by RSuS can be controlled by the availability of fructose and UDP, depending on the metabolic status of a tissue.
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