ReviewMicroscopy (Oxford, England)2016
Two promising future developments of cryo-EM: capturing short-lived states and mapping a continuum of states of a macromolecule.
Review in Microscopy (Oxford, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Article
- Conventional Electron Microscopy, Cryogenic Electron Microscopy, and Cryogenic Electron Tomography of Viruses.Sub-cellular biochemistry · 2024Review
- An RNA excited conformational state at atomic resolution.Nature communications · 2023Article
- Kinetic Resolution of the Atomic 3D Structures Formed by Ground and Excited Conformational States in an RNA Dynamic Ensemble.Journal of the American Chemical Society · 2023Article
- Time resolution in cryo-EM using a novel PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling.bioRxiv : the preprint server for biology · 2023Article
- Exploring the Structural Variability of Dynamic Biological Complexes by Single-Particle Cryo-Electron Microscopy.Micromachines · 2022Review
- Microsecond melting and revitrification of cryo samples: protein structure and beam-induced motion.Acta crystallographica. Section D, Structural biology · 2022Article
- Vanilloid-dependent TRPV1 opening trajectory from cryoEM ensemble analysis.Nature communications · 2022Article
- Recent Technical Advances in Sample Preparation for Single-Particle Cryo-EM.Frontiers in molecular biosciences · 2022Review
- Structure determination of GPCRs: cryo-EM compared with X-ray crystallography.Biochemical Society transactions · 2021Review
- Microsecond melting and revitrification of cryo samples.Structural dynamics (Melville, N.Y.) · 2021Article
- Integrative approaches in cryogenic electron microscopy: Recent advances in structural biology and future perspectives.iScience · 2021Review
- POLARIS: Path of Least Action Analysis on Energy Landscapes.Journal of chemical information and modeling · 2020Article
- Advances in methods for atomic resolution macromolecular structure determination.F1000Research · 2020Review
- Exploring the Nature of Cationic Blocker Recognition by the Anthrax Toxin Channel.Biophysical journal · 2019Article
- Structural dynamics of protein S1 on the 70S ribosome visualized by ensemble cryo-EM.Methods (San Diego, Calif.) · 2018Article
- Time-resolved cryo-electron microscopy: Recent progress.Journal of structural biology · 2017Review
- The potential use of single-particle electron microscopy as a tool for structure-based inhibitor design.Acta crystallographica. Section D, Structural biology · 2017Article
- A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM.Structure (London, England : 1993) · 2017Article
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Abstract
The capabilities and application range of cryogenic electron microscopy (cryo-EM) method have expanded vastly in the last two years, thanks to the advances provided by direct detection devices and computational classification tools. We take this review as an opportunity to sketch out promising developments of cryo-EM in two important directions: (i) imaging of short-lived states (10-1000 ms) of biological molecules by using time-resolved cryo-EM, particularly the mixing-spraying method and (ii) recovering an entire continuum of coexisting states from the same sample by employing a computational technique called manifold embedding. It is tempting to think of combining these two methods, to elucidate the way the states of a molecular machine such as the ribosome branch and unfold. This idea awaits further developments of both methods, particularly by increasing the data yield of the time-resolved cryo-EM method and by developing the manifold embedding technique into a user-friendly workbench.
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