ArticleApplied and environmental microbiology2016
Genus-Specific Primers for Study of Fusarium Communities in Field Samples.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Revisiting the PCR-Based Molecular Approaches for Mycotoxigenic Fusarium Species Detection and Quantification in Wheat.Comprehensive reviews in food science and food safety · 2026Review
- Article
- Metabarcoding Characterization of Fungal Communities in Spanish Cereals with a Special Focus on Fusarium Species.Microbial ecology · 2026Article
- Forecasting Root Rot Disease through Predictive Microbial Functional Profiling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Invasive Fusariosis Among Patients with Acute Leukemia: A 12-Year Single-Center Experience in a Middle-Income Country.Mycopathologia · 2026Article
- First occurrence of Corynespora cassiicola infecting chia plant in Bangladesh and sensitivity of the pathogen to selected fungicides.PloS one · 2026Article
- Article
- High azole MICs in Fusarium spp.: a key factor in treatment decisions for cancer patients?Revista do Instituto de Medicina Tropical de Sao Paulo · 2025Article
- Potential for the Bio-Detoxification of the Mycotoxins Enniatin B and Deoxynivalenol by Lactic Acid Bacteria andMicroorganisms · 2024Article
- Shifts inMicroorganisms · 2024Article
- A comparison between the role of enniatins and deoxynivalenol in Fusarium virulence on different tissues of common wheat.BMC plant biology · 2024Article
- Development of a PCR-based assay for specific and sensitive detection of Fusarium buharicum from infected okra plant.PloS one · 2024Article
- Crop rotation and native microbiome inoculation restore soil capacity to suppress a root disease.Nature communications · 2023Article
- Article
- First Report of Hemp Fusarium Wilt Caused byPlants (Basel, Switzerland) · 2023Article
- Review
- Brassicaceae Fungi and Chromista Diseases: Molecular Detection and Host–Plant InteractionPlants (Basel, Switzerland) · 2023Review
- EndophyticPlants (Basel, Switzerland) · 2022Article
- Infection timing affects Fusarium poae colonization of bread wheat spikes and mycotoxin accumulation in the grain.Journal of the science of food and agriculture · 2022Article
- Genetic Diversity and Population Structure of Head Blight Disease Causing FungusJournal of fungi (Basel, Switzerland) · 2022Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusarium is a large and diverse genus of fungi of great agricultural and economic importance, containing many plant pathogens and mycotoxin producers. To date, high-throughput sequencing of Fusarium communities has been limited by the lack of genus-specific primers targeting regions with high discriminatory power at the species level. In the present study, we evaluated two Fusarium-specific primer pairs targeting translation elongation factor 1 (TEF1). We also present the new primer pair Fa+7/Ra+6. Mock Fusarium communities reflecting phylogenetic diversity were used to evaluate the accuracy of the primers in reflecting the relative abundance of the species. TEF1 amplicons were subjected to 454 high-throughput sequencing to characterize Fusarium communities. Field samples from soil and wheat kernels were included to test the method on more-complex material. For kernel samples, a single PCR was sufficient, while for soil samples, nested PCR was necessary. The newly developed primer pairs Fa+7/Ra+6 and Fa/Ra accurately reflected Fusarium species composition in mock DNA communities. In field samples, 47 Fusarium operational taxonomic units were identified, with the highest Fusarium diversity in soil. The Fusarium community in soil was dominated by members of the Fusarium incarnatum-Fusarium equiseti species complex, contradicting findings in previous studies. The method was successfully applied to analyze Fusarium communities in soil and plant material and can facilitate further studies of Fusarium ecology.
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