ArticleMethods in molecular biology (Clifton, N.J.)2016
Real-Time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells.
Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Protocol for assessing protein expression levels and localization during fission yeast cytokinesis.STAR protocols · 2026Article
- The MO25 protein Pmo25 functions in contractile ring stability and Sid2 localization during cytokinesis.iScience · 2026Article
- Localization and function of septins are susceptible to epitope tagging.Molecular biology of the cell · 2025Article
- Article
- The Ync13-Rga7-Rng10 complex selectively coordinates secretory vesicle trafficking and secondary septum formation during cytokinesis.PLoS biology · 2025Article
- Localization and function of septins are susceptible to epitope tagging.bioRxiv : the preprint server for biology · 2025Article
- Roles of the MO25 protein Pmo25 in contractile-ring stability and localization of the NDR kinase Sid2 during cytokinesis.bioRxiv : the preprint server for biology · 2025Article
- Article
- Localizations of the septin Spn4 tagged with GFP and mEGFP in fission yeast.microPublication biology · 2025Article
- Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells.Journal of visualized experiments : JoVE · 2023Article
- Membrane dynamics and protein targets of lipid droplet microautophagy during ER stress-induced proteostasis in the budding yeast,Autophagy · 2021Article
- Roles of Mso1 and the SM protein Sec1 in efficient vesicle fusion during fission yeast cytokinesis.Molecular biology of the cell · 2020Article
- Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.Molecular biology of the cell · 2018Article
- Tailor-made PL-UC-C3 nanoparticles for fluorescence/computed tomography imaging-guided cascade amplified photothermal therapy.International journal of nanomedicine · 2018Article
- Roles of the TRAPP-II Complex and the Exocyst in Membrane Deposition during Fission Yeast Cytokinesis.PLoS biology · 2016Article
- Sbg1 Is a Novel Regulator for the Localization of the β-Glucan Synthase Bgs1 in Fission Yeast.PloS one · 2016Article
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4 authors.
Funding
Abstract
Single-cell microscopy provides a powerful tool to visualize cellular and subcellular processes in wild-type and mutant cells by observing fluorescently tagged proteins. Here, we describe three simple methods to visualize fission yeast cells: gelatin slides, coverslip-bottom dishes, and tetrad fluorescence microscopy. These imaging methods and data analysis using free software make it possible to quantify protein localization, dynamics, and concentration with high spatial and temporal resolution. In fission yeast, the actomyosin contractile ring is essential for cytokinesis. We use the visualization and quantification of contractile ring proteins as an example to demonstrate how to use these methods.
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