ArticlePlant molecular biology2016
A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.).
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
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- A New Set of Golden-Gate-Based Organelle Marker Plasmids for Colocalization Studies in Plants.Plants (Basel, Switzerland) · 2022Article
- A New Method for Rapid Subcellular Localization and Gene Function Analysis in Cotton Based on Barley Stripe Mosaic Virus.Plants (Basel, Switzerland) · 2022Article
- Genome-wide, evolutionary, and functional analyses of ascorbate peroxidase (APX) family in Poaceae species.Genetics and molecular biology · 2022Article
- Validation of a high-confidence regulatory network for gene-to-NUE phenotype in field-grown rice.Frontiers in plant science · 2022Article
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- Organelle Visualization With Multicolored Fluorescent Markers in Bamboo.Frontiers in plant science · 2021Article
- Fluorescent reporters for functional analysis in rice leaves.Plant direct · 2020Article
- Green Fluorescent Protein- andFrontiers in plant science · 2019Article
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- Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice.Rice (New York, N.Y.) · 2018Article
- Peroxisome Function, Biogenesis, and Dynamics in Plants.Plant physiology · 2018Review
- Visualization of Multicolored in vivo Organelle Markers for Co-Localization Studies in Oryza sativa.Molecules and cells · 2017Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; however, useful and reliable fluorescent organelle marker lines are lacking in the monocot model rice. Here, we developed eight different GFP-based organelle markers in transgenic rice and created a set of DsRed-based gateway vectors for combining with the marker lines. Two mitochondrial-localized rice ascorbate peroxidase genes fused to DsRed and successfully co-localized with mitochondrial-targeted marker lines verified the practical use of this system. The co-localization of GFP-fusion marker lines and DsRed-fusion proteins provide a convenient platform for in vivo or in vitro analysis of subcellular localization of rice proteins.
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