Evidence map›Paper›PMID 26519030›Full record

ArticleAmerican journal of physiology. Heart and circulatory physiology2016

MPST but not CSE is the primary regulator of hydrogen sulfide production and function in the coronary artery.

Maggie M Kuo, Dae Hee Kim, Sandeep Jandu, Yehudit Bergman, Siqi Tan, Huilei Wang, Deepesh R Pandey, Theodore P Abraham, Artin A Shoukas, Dan E Berkowitz and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Heart and circulatory physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
4.0field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 55 citations in OpenAlex.

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  16. Hydrogen sulfide signaling in mitochondria and disease.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019
    Review
  17. Frontiers in microbiology · 2019
    Article
  18. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Maggie M KuoDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland;
Dae Hee KimDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland; Department of Anethesiology and Pain Medicine, Ajou University School of Medicine, Suwon, Korea.
Sandeep JanduDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland;
Yehudit BergmanDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland;
Siqi TanDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland;
Huilei WangDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland;
Deepesh R PandeyDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland;
Theodore P AbrahamDepartment of Medicine (Cardiology), Johns Hopkins University, Baltimore, Maryland; and.
Artin A ShoukasDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland;
Dan E BerkowitzDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland;
Lakshmi SanthanamDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland; Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland; LSantha1@jhmi.edu.
Johns Hopkins Medicine · USJohns Hopkins University · US

Funding

Transglutaminase 2 S-Nitrosylation: Role in Age-Related Vascular StiffnessR01HL105296 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI BERKOWITZ, DAN E · 2010 to 2013
$1.6M
NHLBI NIH HHS 1R01-HL-105296-01
6 · The paper itself

Abstract

Hydrogen sulfide (H2S) has emerged as an important gasotransmitter in the vasculature. In this study, we tested the hypothesis that H2S contributes to coronary vasoregulation and evaluated the physiological relevance of two sources of H2S, namely, cystathionine-γ-lyase (CSE) and 3-mercaptypyruvate sulfertransferase (MPST). MPST was detected in human coronary artery endothelial cells as well as rat and mouse coronary artery; CSE was not detected in the coronary vasculature. Rat coronary artery homogenates produced H2S through the MPST pathway but not the CSE pathway in vitro. In vivo coronary vasorelaxation response was similar in CSE knockout mice, wild-type mice (WT), and WT mice treated with the CSE inhibitor propargylglycine, suggesting that CSE-produced H2S does not have a significant role in coronary vasoregulation in vivo. Ex vivo, the MPST substrate 3-mercaptopyruvate (3-MP) and H2S donor sodium hydrosulfide (NaHS) elicited similar coronary vasoreactivity responses. Pyruvate did not have any effects on vasoreactivity. The vasoactive effect of H2S appeared to be nitric oxide (NO) dependent: H2S induced coronary vasoconstriction in the presence of NO and vasorelaxation in its absence. Maximal endothelial-dependent relaxation was intact after 3-MP and NaHS induced an increase in preconstriction tone, suggesting that endothelial NO synthase activity was not significantly inhibited. In vitro, H2S reacted with NO, which may, in part explain the vasoconstrictive effects of 3-MP and NaHS. Taken together, these data show that MPST rather than CSE generates H2S in coronary artery, mediating its effects through direct modulation of NO. This has important implications for H2S-based therapy in healthy and diseased coronary arteries.

Indexed as

AnimalsCells, CulturedCoronary VesselsCystathionine gamma-LyaseDose-Response Relationship, DrugEnzyme InhibitorsHumansHydrogen SulfideKineticsMaleMice, KnockoutNitric OxideRats, Sprague-DawleySignal TransductionSulfurtransferasesVasodilation3-mercaptopyruvate sulphurtransferaseCystathionine gamma-LyaseEnzyme InhibitorsHydrogen SulfideNitric OxideSulfurtransferasesVasodilator Agents3-mercaptopyruvate sulfurtransferasecoronary tonecoronary vasoregulationcystathionine-γ-lyasehydrogen sulfide

Identifiers

PMID26519030
PMCPMC4796461
OpenAlexW1884763982

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.