ArticleThe Journal of biological chemistry2016
Krüppel Homolog 1 Inhibits Insect Metamorphosis via Direct Transcriptional Repression of Broad-Complex, a Pupal Specifier Gene.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 137 citations in OpenAlex.
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- Duplicated paralog of insulin receptor functions distinctively in locust reproduction for high fecundity.Insect science · 2026Article
- Transient remodeling of gut metabolism supports juvenile growth and adult fitness in Drosophila.Nature communications · 2026Article
- Prioritising research on endocrine disruption in the marine environment: a global perspective.Biological reviews of the Cambridge Philosophical Society · 2026Review
- SUMOylation modulates the dual functions of Krüppel homolog 1 in transcriptional regulation of Broad-Complex expression.Journal of advanced research · 2025Article
- 20E-inducedProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Chromatin Accessibility Dynamics Reveal Conserved Transcriptional Regulatory Networks During Insect Metamorphosis inBiology · 2025Article
- Transcriptomic Characterization of miRNAs inGenes · 2025Article
- Histone deacetylases synergistically regulate juvenile hormone signaling in the yellow fever mosquito, Aedes aegypti.Insect biochemistry and molecular biology · 2025Article
- Chronologically inappropriate morphogenesis (Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Methoprene-Tolerant (Met) Acts as Methyl Farnesoate Receptor to Regulate Larva Metamorphosis in Mud Crab,International journal of molecular sciences · 2024Article
- Schlank orchestrates insect developmental transition by switching H3K27 acetylation to trimethylation in the prothoracic gland.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
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- Overexpression ofInternational journal of molecular sciences · 2023Article
- Restoration of energy homeostasis under oxidative stress: Duo synergistic AMPK pathways regulating arginine kinases.PLoS genetics · 2023Article
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- Exorista sorbillans (Diptera: Tachinidae) parasitism shortens host larvae growth duration by regulating ecdysone and juvenile hormone titers in Bombyx mori (Lepidoptera: Bombycidae).Journal of insect science (Online) · 2023Article
- Review
5 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Broad-Complex gene (BR-C) encodes transcription factors that dictate larval-pupal metamorphosis in insects. The expression of BR-C is induced by molting hormone (20-hydroxyecdysone (20E)), and this induction is repressed by juvenile hormone (JH), which exists during the premature larval stage. Krüppel homolog 1 gene (Kr-h1) has been known as a JH-early inducible gene responsible for repression of metamorphosis; however, the functional relationship between Kr-h1 and repression of BR-C has remained unclear. To elucidate this relationship, we analyzed cis- and trans elements involved in the repression of BR-C using a Bombyx mori cell line. In the cells, as observed in larvae, JH induced the expression of Kr-h1 and concurrently suppressed 20E-induced expression of BR-C. Forced expression of Kr-h1 repressed the 20E-dependent activation of the BR-C promoter in the absence of JH, and Kr-h1 RNAi inhibited the JH-mediated repression, suggesting that Kr-h1 controlled the repression of BR-C. A survey of the upstream sequence of BR-C gene revealed a Kr-h1 binding site (KBS) in the BR-C promoter. When KBS was deleted from the promoter, the repression of BR-C was abolished. Electrophoresis mobility shift demonstrated that two Kr-h1 molecules bound to KBS in the BR-C promoter. Based on these results, we conclude that Kr-h1 protein molecules directly bind to the KBS sequence in the BR-C promoter and thereby repress 20E-dependent activation of the pupal specifier, BR-C. This study has revealed a considerable portion of the picture of JH signaling pathways from the reception of JH to the repression of metamorphosis.
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