ArticleBMC microbiology2015
Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species.
Article in BMC microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Shaping the future ofJournal of medical microbiology · 2025Review
- Beyond serology: saccharide profiling enables identification of antigenically similarFrontiers in molecular biosciences · 2025Article
- Isolation and characterization of Leptospira licerasiae in Austrian swine - a first-time case report in Europe.BMC veterinary research · 2024Article
- Cellular Pathophysiology of Leptospirosis: Role of Na/K-ATPase.Microorganisms · 2023Review
- Horizontal transfer of theLife science alliance · 2023Article
- Lipid A structural diversity among members of the genusFrontiers in microbiology · 2023Article
- Host and Species-Specificities of Pattern Recognition Receptors Upon Infection WithFrontiers in cellular and infection microbiology · 2022Review
- Pathogenic Leptospires Limit Dendritic Cell Activation Through Avoidance of TLR4 and TRIF Signaling.Frontiers in immunology · 2022Article
- Applied Proteomics in 'One Health'.Proteomes · 2021Review
- Comparative Genomic Analysis and a Novel Set of Missense Mutation of theFrontiers in microbiology · 2021Article
- Leptospiral LPS escapes mouse TLR4 internalization and TRIF‑associated antimicrobial responses through O antigen and associated lipoproteins.PLoS pathogens · 2020Article
- Comparative genomic analysis of Chinese human leptospirosis vaccine strain and circulating isolate.Human vaccines & immunotherapeutics · 2020Article
- Leptospirosis is an invasive infectious and systemic inflammatory disease.Biomedical journal · 2020Review
- Genomic analysis of Leptospira interrogans serovar Paidjan and Dadas isolates from carrier dogs and comparative genomic analysis to detect genes under positive selection.BMC genomics · 2019Article
- An in silico approach towards identification of novel drug targets in pathogenic species of Leptospira.PloS one · 2019Article
- ThreeFrontiers in microbiology · 2019Article
- Experimental Infection ofThe American journal of tropical medicine and hygiene · 2018Article
- A novel Fas-binding outer membrane protein and lipopolysaccharide of Leptospira interrogans induce macrophage apoptosis through the Fas/FasL-caspase-8/-3 pathway.Emerging microbes & infections · 2018Article
- Comparison of microbiota and allergen profile in house dust from homes of allergic and non-allergic subjects- results from the GUSTO study.The World Allergy Organization journal · 2018Article
- Comparison of lipopolysaccharides composition of two different strains of Helicobacter pylori.BMC microbiology · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLipopolysaccharides (LPS) are complex, amphipathic biomolecules that constitute the major surface component of Gram-negative bacteria. Leptospira, unlike other human-pathogenic spirochetes, produce LPS, which is fundamental to the taxonomy of the genus, involved in host-adaption and also the target of diagnostic antibodies. Despite its significance, little is known of Leptospira LPS composition and carbohydrate structure among different serovars.
resultsLPS from Leptospira interrogans serovar Copenhageni strain L1-130, a pathogenic species, and L. licerasiae serovar Varillal strain VAR 010, an intermediately pathogenic species, were studied. LPS prepared from aqueous and phenol phases were analyzed separately. L. interrogans serovar Copenhageni has additional sugars not found in L. licerasiae serovar Varillal, including fucose (2.7%), a high amount of GlcNAc (12.3%), and two different types of dideoxy HexNAc. SDS-PAGE indicated that L. interrogans serovar Copenhageni LPS had a far higher molecular weight and complexity than that of L. licerasiae serovar Varillal. Chemical composition showed that L. interrogans serovar Copenhageni LPS has an extended O-antigenic polysaccharide consisting of sugars, not present in L. licerasiae serovar Varillal. Arabinose, xylose, mannose, galactose and L-glycero-D-mannoheptose were detected in both the species. Fatty acid analysis by gas chromatography-mass spectrometry (GC-MS) showed the presence of hydroxypalmitate (3-OH-C16:0) only in L. interrogans serovar Copenhageni. Negative staining electron microscopic examination of LPS showed different filamentous morphologies in L. interrogans serovar Copenhageni vs. L. licerasiae serovar Varillal.
conclusionsThis comparative biochemical analysis of pathogenic and intermediately pathogenic Leptospira LPS reveals important carbohydrate and lipid differences that underlie future work in understanding the mechanisms of host-adaptation, pathogenicity and vaccine development in leptospirosis.
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