Evidence map›Paper›PMID 26518300›Full record

ArticleJournal of dental research2016

MicroRNA-138 Inhibits Periodontal Progenitor Differentiation under Inflammatory Conditions.

X Zhou, X Luan, Z Chen, M Francis, G Gopinathan, W Li, X Lu, S Li, C Wu, T G H Diekwisch

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of dental research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 1 pooled it
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. MicroRNAs: Harbingers and shapers of periodontal inflammation.Seminars in cell & developmental biology · 2022
    Review
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. MicroRNAs in orthopaedic research: Disease associations, potential therapeutic applications, and perspectives.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018
    Review
  19. Article
  20. microRNA Regulation of Skeletal Development.Current osteoporosis reports · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

X ZhouDepartment of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
X LuanDepartment of Oral Biology, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
Z ChenDepartment of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
M FrancisDepartment of Oral Biology, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
G GopinathanDepartment of Oral Biology, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
W LiDepartment of Oral Biology, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
X LuDepartment of Oral Biology, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
S LiDepartment of Oral Biology, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
C WuDepartment of Pediatric Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
T G H DiekwischDepartment of Periodontics, Baylor College of Dentistry, Dallas, TX, USA diekwisch@bcd.tamhsc.edu.
University of Illinois Chicago · USBaylor University · US

Funding

Small Molecule Microenvironment Design for Craniofacial Bone Regeneration and EngineeringR01DE019463 · NIDCR · UNIVERSITY OF ROCHESTER · PI LUAN, XIANGHONG · 2009 to 2021
$2.8M
MicroRNA determinants of oral cancer detectionR03CA171436 · NCI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI ZHOU, XIAOFENG · 2013 to 2014
$157k
NCI NIH HHS CA171436NCI NIH HHS R03 CA171436NIDCR NIH HHS DE019463-01NIDCR NIH HHS R01 DE019463
6 · The paper itself

Abstract

Inflammatory conditions as they occur during periodontal disease often result in decreased alveolar bone levels and a loss of connective tissue homeostasis. Here we have focused on the effect of microRNA-138 (miR-138) as a potential regulator of periodontal stem cells as they affect homeostasis during inflammatory conditions. Our data indicate that miR-138 was significantly upregulated in our periodontal disease animal model. Interaction of miR-138 with a predicted targeting site on the osteocalcin (OC) promoter resulted in a 3.7-fold reduction of luciferase activity in promoter assays compared with controls; and miR-138 overexpression in periodontal progenitors significantly inhibited OC (3.4-fold), Runx2 (2.8-fold), and collagen I (2.6-fold). Moreover, treatment with inflammatory modulators such as interleukin (IL)-6 and lipopolysaccharide (LPS) resulted in a significant 2.2-fold (IL-6) or 1.9-fold (LPS) increase in miR-138 expression, while OC and Runx2 expression was significantly decreased as a result of treatment with each inflammatory mediator. Further defining the role of miR-138 in the OC-mediated control of mineralization, we demonstrated that the LPS-induced downregulation of OC expression was partially reversed after miR-138 knockdown. LPS, miR-138 mimic, and OC small interfering RNA inhibited osteoblast differentiation marker alkaline phosphatase activity, while miR-138 inhibitor and OC protein addition enhanced alkaline phosphatase activity. Supporting the role of OC as an essential modulator of osteoblast differentiation, knockdown of miR-138 or addition of OC protein partially rescued alkaline phosphatase activity in periodontal ligament (PDL) cells subjected to LPS treatment. Our data establish miR-138 inhibitor as a potential therapeutic agent for the prevention of the bone loss associated with advanced periodontal disease.

Indexed as

Alkaline PhosphataseAnimalsCell DifferentiationCells, CulturedCollagen Type ICore Binding Factor Alpha 1 SubunitDisease Models, AnimalGene Knockdown TechniquesHomeostasisHumansInflammation MediatorsInterleukin-6LipopolysaccharidesLuciferasesMicroRNAsOsteoblastsAlkaline PhosphataseCollagen Type ICore Binding Factor Alpha 1 SubunitInflammation MediatorsInterleukin-6LipopolysaccharidesLuciferasesMicroRNAsMIRN138 microRNA, ratOsteocalcinRNA, Small InterferingRunx2 protein, ratalveolar bone lossosteocalcinosteogenesisperiodontal diseasesperiodontal ligamentstem cells

Identifiers

PMID26518300
PMCPMC4720956
OpenAlexW1828164527

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.