ArticleIranian biomedical journal2016
A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System.
Article in Iranian biomedical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Expressing of Recombinant VEGFR2-specific Nanobody in Baculovirus Expression System.Iranian journal of biotechnology · 2021Article
- Expression of a New Recombinant Collagenase Protein ofIranian journal of biotechnology · 2019Article
- Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody.Iranian journal of basic medical sciences · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBaculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells.
methodsThe aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells.
resultsExpression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis.
conclusionThe application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.
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