ArticlePloS one2015
Dispersion Behaviour of Silica Nanoparticles in Biological Media and Its Influence on Cellular Uptake.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Caveolin-initiated macropinocytosis is required for efficient silica nanoparticles' transcytosis across the alveolar epithelial barrier.Scientific reports · 2022Article
- How the Physicochemical Properties of Manufactured Nanomaterials Affect Their Performance in Dispersion and Their Applications in Biomedicine: A Review.Nanomaterials (Basel, Switzerland) · 2022Review
- Lactose-Gated Mesoporous Silica Particles for Intestinal Controlled Delivery of Essential Oil Components: An In Vitro and In Vivo Study.Pharmaceutics · 2021Article
- Serum Lowers Bioactivity and Uptake of Synthetic Amorphous Silica by Alveolar Macrophages in a Particle Specific Manner.Nanomaterials (Basel, Switzerland) · 2021Article
- Quantitative Particle Uptake by Cells as Analyzed by Different Methods.Angewandte Chemie (International ed. in English) · 2020Review
- Extra-Small Gold Nanospheres Decorated With a Thiol Functionalized Biodegradable and Biocompatible Linear Polyamidoamine as Nanovectors of Anticancer Molecules.Frontiers in bioengineering and biotechnology · 2020Article
- Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy.Nanomaterials (Basel, Switzerland) · 2019Article
- Recent advances in the application of silica nanostructures for highly improved water treatment: a review.Environmental science and pollution research international · 2019Article
- Mapping of the available standards against the regulatory needs for nanomedicines.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2019Review
- Assessment of in vitro particle dosimetry models at the single cell and particle level by scanning electron microscopy.Journal of nanobiotechnology · 2018Article
- Article
- In Vitro and In Vivo Short-Term Pulmonary Toxicity of Differently Sized Colloidal Amorphous SiO₂.Nanomaterials (Basel, Switzerland) · 2018Article
- Silica nanoparticle stability in biological media revisited.Scientific reports · 2018Article
- Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles.Bioengineering (Basel, Switzerland) · 2018Article
- Inner-View of Nanomaterial Incited Protein Conformational Changes: Insights into Designable Interaction.Research (Washington, D.C.) · 2018Review
- The agglomeration state of nanoparticles can influence the mechanism of their cellular internalisation.Journal of nanobiotechnology · 2017Article
- Current Challenges toward In Vitro Cellular Validation of Inorganic Nanoparticles.Bioconjugate chemistry · 2017Review
- Quantification of the cellular dose and characterization of nanoparticle transport during in vitro testing.Particle and fibre toxicology · 2016Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Given the increasing variety of manufactured nanomaterials, suitable, robust, standardized in vitro screening methods are needed to study the mechanisms by which they can interact with biological systems. The in vitro evaluation of interactions of nanoparticles (NPs) with living cells is challenging due to the complex behaviour of NPs, which may involve dissolution, aggregation, sedimentation and formation of a protein corona. These variable parameters have an influence on the surface properties and the stability of NPs in the biological environment and therefore also on the interaction of NPs with cells. We present here a study using 30 nm and 80 nm fluorescently-labelled silicon dioxide NPs (Rubipy-SiO2 NPs) to evaluate the NPs dispersion behaviour up to 48 hours in two different cellular media either supplemented with 10% of serum or in serum-free conditions. Size-dependent differences in dispersion behaviour were observed and the influence of the living cells on NPs stability and deposition was determined. Using flow cytometry and fluorescence microscopy techniques we studied the kinetics of the cellular uptake of Rubipy-SiO2 NPs by A549 and CaCo-2 cells and we found a correlation between the NPs characteristics in cell media and the amount of cellular uptake. Our results emphasize how relevant and important it is to evaluate and to monitor the size and agglomeration state of nanoparticles in the biological medium, in order to interpret correctly the results of the in vitro toxicological assays.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.