ArticleInternational journal of molecular sciences2015
De Novo Sequencing and Analysis of the Safflower Transcriptome to Discover Putative Genes Associated with Safflor Yellow in Carthamus tinctorius L.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Screening and functional identification of CtMYB2 as a positive regulator of flavonoid biosynthesis in safflower (Carthamus tinctorius L.).BMC plant biology · 2026Article
- Total Flavonoids fromInternational journal of molecular sciences · 2026Article
- Research Progress of Genomics Applications in Secondary Metabolites of Medicinal Plants: A Case Study in Safflower.International journal of molecular sciences · 2025Review
- Transcriptome and metabolome analyses revealed the main profiles contributing the mild aroma characteristics ofFrontiers in plant science · 2025Article
- Molecular Characterization of anMolecules (Basel, Switzerland) · 2022Article
- Transcriptome and Metabolome Analyses Reveal Differences in Terpenoid and Flavonoid Biosynthesis inFrontiers in plant science · 2022Article
- Genome-wide analysis and transcriptional reprogrammings of MYB superfamily revealed positive insights into abiotic stress responses and anthocyanin accumulation in Carthamus tinctorius L.Molecular genetics and genomics : MGG · 2022Article
- Temporal transcriptome profiling of developing seeds reveals candidate genes involved in oil accumulation in safflower (Carthamus tinctorius L.).BMC plant biology · 2021Article
- Full-Length Transcriptome Sequencing Provides Insights into Flavonoid Biosynthesis inLife (Basel, Switzerland) · 2021Article
- Genome-wide identification and expression analysis of bZIP gene family in Carthamus tinctorius L.Scientific reports · 2020Article
- Alternative Splicing Regulation of Anthocyanin Biosynthesis inG3 (Bethesda, Md.) · 2020Article
- Transcriptome Sequencing and Chemical Analysis Reveal the Formation Mechanism of White Florets inPlants (Basel, Switzerland) · 2020Article
- A Metabolic Perspective and Opportunities in Pharmacologically Important Safflower.Metabolites · 2020Review
- Article
- Integrating molecular characterization and metabolites profile revealed CtCHI1's significant role in Carthamus tinctorius L.BMC plant biology · 2019Article
- Molecular cloning and functional characterization of chalcone isomerase from Carthamus tinctorius.AMB Express · 2019Article
- De novo sequencing of tree peony (Paeonia suffruticosa) transcriptome to identify critical genes involved in flowering and floral organ development.BMC genomics · 2019Article
- Genome-Wide Identification, Expression Analysis, and Subcellular Localization ofInternational journal of molecular sciences · 2019Article
- Transcriptome Sequencing and Biochemical Analysis of Perianths and Coronas Reveal Flower Color Formation inInternational journal of molecular sciences · 2018Article
- Full-length transcriptome sequences and the identification of putative genes for flavonoid biosynthesis in safflower.BMC genomics · 2018Article
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Safflower (Carthamus tinctorius L.), an important traditional Chinese medicine, is cultured widely for its pharmacological effects, but little is known regarding the genes related to the metabolic regulation of the safflower's yellow pigment. To investigate genes related to safflor yellow biosynthesis, 454 pyrosequencing of flower RNA at different developmental stages was performed, generating large databases.In this study, we analyzed 454 sequencing data from different flowering stages in safflower. In total, 1,151,324 raw reads and 1,140,594 clean reads were produced, which were assembled into 51,591 unigenes with an average length of 679 bp and a maximum length of 5109 bp. Among the unigenes, 40,139 were in the early group, 39,768 were obtained from the full group and 28,316 were detected in both samples. With the threshold of "log2 ratio ≥ 1", there were 34,464 differentially expressed genes, of which 18,043 were up-regulated and 16,421 were down-regulated in the early flower library. Based on the annotations of the unigenes, 281 pathways were predicted. We selected 12 putative genes and analyzed their expression levels using quantitative real time-PCR. The results were consistent with the 454 sequencing results. In addition, the expression of chalcone synthase, chalcone isomerase and anthocyanidin synthase, which are involved in safflor yellow biosynthesis and safflower yellow pigment (SYP) content, were analyzed in different flowering periods, indicating that their expression levels were related to SYP synthesis. Moreover, to further confirm the results of the 454 pyrosequencing, full-length cDNA of chalcone isomerase (CHI) and anthocyanidin synthase (ANS) were cloned from safflower petal by RACE (Rapid-amplification of cDNA ends) method according to fragment of the transcriptome.
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