ArticleJournal of the American Society of Nephrology : JASN2016
Functional Human Podocytes Generated in Organoids from Amniotic Fluid Stem Cells.
Article in Journal of the American Society of Nephrology : JASN, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 57 citations in OpenAlex.
- Enhancing maturity in 3D kidney micro-tissues through clonogenic cell combinations and endothelial integration.Journal of cellular and molecular medicine · 2024Article
- Body fluid-derived stem cells - an untapped stem cell source in genitourinary regeneration.Nature reviews. Urology · 2023Review
- Patient-derived podocyte spheroids reveal new insights into the etiopathogenesis of Alport syndrome.Frontiers in cell and developmental biology · 2023Article
- Kidney organoids: current knowledge and future directions.Cell and tissue research · 2022Review
- Beneficial Impact of Interspecies Chimeric Renal Organoids Against a Xenogeneic Immune Response.Frontiers in immunology · 2022Article
- Unravelling the Role of PAX2 Mutation in Human Focal Segmental Glomerulosclerosis.Biomedicines · 2021Article
- Modelling Renal Filtration and Reabsorption Processes in a Human Glomerulus and Proximal Tubule Microphysiological System.Micromachines · 2021Article
- Bioengineered Kidney Models: Methods and Functional Assessments.Function (Oxford, England) · 2021Review
- The Role of NF-kB in the Downregulation of Organic Cation Transporter 2 Expression and Renal Cation Secretion in Kidney Disease.Frontiers in medicine · 2021Article
- Perinatal Derivatives: Where Do We Stand? A Roadmap of the Human Placenta and Consensus for Tissue and Cell Nomenclature.Frontiers in bioengineering and biotechnology · 2020Review
- Kidney Organoids as Disease Models: Strengths, Weaknesses and Perspectives.Frontiers in physiology · 2020Review
- Molecular stratification of idiopathic nephrotic syndrome.Nature reviews. Nephrology · 2019Review
- Transplantation of Mouse Induced Pluripotent Stem Cell-Derived Podocytes in a Mouse Model of Membranous Nephropathy Attenuates Proteinuria.Scientific reports · 2019Article
- The moral status of cerebral organoids.Regenerative therapy · 2019Review
- Engineering the vasculature of decellularized rat kidney scaffolds using human induced pluripotent stem cell-derived endothelial cells.Scientific reports · 2019Article
- Directed Differentiation of Human Pluripotent Stem Cells to Podocytes under Defined Conditions.Scientific reports · 2019Article
- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Engineered Kidney Tubules for Modeling Patient-Specific Diseases and Drug Discovery.EBioMedicine · 2018Article
- Amniotic fluid cells: current progress and emerging challenges in renal regeneration.Pediatric nephrology (Berlin, Germany) · 2018Review
- The presence of human mesenchymal stem cells of renal origin in amniotic fluid increases with gestational time.Stem cell research & therapy · 2018Article
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Generating kidney organoids using human stem cells could offer promising prospects for research and therapeutic purposes. However, no cell-based strategy has generated nephrons displaying an intact three-dimensional epithelial filtering barrier. Here, we generated organoids using murine embryonic kidney cells, and documented that these tissues recapitulated the complex three-dimensional filtering structure of glomerular slits in vivo and accomplished selective glomerular filtration and tubular reabsorption. Exploiting this technology, we mixed human amniotic fluid stem cells with mouse embryonic kidney cells to establish three-dimensional chimeric organoids that engrafted in vivo and grew to form vascularized glomeruli and tubular structures. Human cells contributed to the formation of glomerular structures, differentiated into podocytes with slit diaphragms, and internalized exogenously infused BSA, thus attaining in vivo degrees of specialization and function unprecedented for donor stem cells. In conclusion, human amniotic fluid stem cell chimeric organoids may offer new paths for studying renal development and human podocyte disease, and for facilitating drug discovery and translational research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.