ArticleJournal of experimental botany2016
MutS HOMOLOG1 silencing mediates ORF220 substoichiometric shifting and causes male sterility in Brassica juncea.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- A Systematic Review and Developmental Perspective on Origin of CMS Genes in Crops.International journal of molecular sciences · 2024Pooled it
- Harnessing mitochondrial genome editing for crop improvement: principles and applications.aBIOTECH · 2026Review
- Cytoplasmic inheritance: The transmission of plastid and mitochondrial genomes across cells and generations.Plant physiology · 2025Review
- Identification, cloning and characterization of AcMSH1 from Onion (Allium cepa L.).Molecular biology reports · 2023Article
- Genic male and female sterility in vegetable crops.Horticulture research · 2023Article
- Long-read sequencing characterizes mitochondrial and plastid genome variants in Arabidopsis msh1 mutants.The Plant journal : for cell and molecular biology · 2022Article
- The Development of Mitochondrial Gene Editing Tools and Their Possible Roles in Crop Improvement for Future Agriculture.Advanced genetics (Hoboken, N.J.) · 2022Review
- Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile LineFrontiers in plant science · 2021Article
- The Mitochondrial Genome Assembly of Fennel (International journal of molecular sciences · 2020Article
- Organellar protein multi-functionality and phenotypic plasticity in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Many Facets of Dynamic Plasticity in Plants.Cold Spring Harbor perspectives in biology · 2019Review
- Construction of restorer lines and molecular mapping for restorer gene of hau cytoplasmic male sterility in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Identification and Mapping of a New Soybean Male-Sterile Gene,Frontiers in plant science · 2019Article
- Characterization of genes specific to sua-CMS in Nicotiana tabacum.Plant cell reports · 2018Article
- Transcript levels of orf288 are associated with the hau cytoplasmic male sterility system and altered nuclear gene expression in Brassica juncea.Journal of experimental botany · 2018Article
- MutS-Homolog2 silencing generates tetraploid meiocytes in tomato (Plant direct · 2018Article
- Morphological and genetic characterization of a new cytoplasmic male sterility system (oxa CMS) in stem mustard (Brassica juncea).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Stress-responsive pathways and small RNA changes distinguish variable developmental phenotypes caused by MSH1 loss.BMC plant biology · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoplasmic male sterility (CMS) has consistently been associated with the expression of mitochondrial open reading frames (ORFs) that arise from genomic rearrangements. Spontaneous fertility reversion in CMS has been observed in several cases, but a clear understanding of fertility reversion controlled by nuclear genetic influences has been lacking. Here, we identified spontaneous fertile revertant lines for Brassica juncea CMS cytoplasm in which the mitochondrial genome has undergone substoichiometric shifting (SSS) to suppress ORF220 copy number. We placed ORF220, with or without a mitochondrial targeting presequence, under the control of the CaMV35S and AP3 promoters in Arabidopsis to confirm that ORF220 causes male sterility when mitochondrially localized. We found that copy number of the ORF220 gene was altered under conditions that suppress MSH1, a nuclear gene that controls illegitimate recombination in plant mitochondria. MSH1-RNAi lines with increased ORF220 copy number were male sterile compared with wild type. We found that a wide range of genes involved in anther development were up- and down-regulated in revertant and MSH1-RNAi lines, respectively. The system that we have developed offers valuable future insight into the interplay of MSH1 and SSS in CMS induction and fertility reversion as a mediator of nuclear-mitochondrial crosstalk.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.