Evidence map›Paper›PMID 26515110›Full record

ArticleNature communications2015

Resident c-kit(+) cells in the heart are not cardiac stem cells.

Nishat Sultana, Lu Zhang, Jianyun Yan, Jiqiu Chen, Weibin Cai, Shegufta Razzaque, Dongtak Jeong, Wei Sheng, Lei Bu, Mingjiang Xu and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 182 papers.

0numbers the graph read from it
0cells of the map it votes in
182citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

182 citing papers in PubMed.

  1. Trial
  2. Review
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  7. Review
  8. Pluripotent stem cell-based approaches for heart repair and the potential of genetic modifications.Molecular therapy : the journal of the American Society of Gene Therapy · 2025
    Review
  9. Heart-derived endogenous stem cells.Molecular biology reports · 2025
    Review
  10. Review
  11. Review
  12. Article
  13. Article
  14. Review
  15. Article
  16. Review
  17. Stem cells-derived exosomes as cardiac regenerative agents.International journal of cardiology. Heart & vasculature · 2024
    Review
  18. Review
  19. Cell Therapy in the Treatment of Coronary Heart Disease.International journal of molecular sciences · 2023
    Review
  20. Article

122 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Nishat SultanaDepartment of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
Lu ZhangDepartment of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
Jianyun YanDepartment of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
Jiqiu ChenDepartment of Medicine, Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
Weibin CaiDepartment of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
Shegufta RazzaqueDepartment of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
Dongtak JeongDepartment of Medicine, Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
Wei ShengCardiovascular Center, Children's Hospital of Fudan University, Shanghai 201102, China.
Lei BuLeon H. Charney Division of Cardiology, Department of Medicine, New York University School of Medicine, New York, New York 10016, USA.
Mingjiang XuDepartment of Biochemistry and Molecular Biology, Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, Florida 33136, USA.
Guo-Ying HuangCardiovascular Center, Children's Hospital of Fudan University, Shanghai 201102, China.
Roger J HajjarDepartment of Medicine, Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
Bin ZhouDepartment of Genetics, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA.
Anne MoonWeis Center for Research, Geisinger Clinic, Danville, Pennsylvania 17822, USA.
Chen-Leng CaiDepartment of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Identifying a bona fide population of cardiac stem cells (CSCs) is a critical step for developing cell-based therapies for heart failure patients. Previously, cardiac c-kit(+) cells were reported to be CSCs with a potential to become myocardial, endothelial and smooth muscle cells in vitro and after cardiac injury. Here we provide further insights into the nature of cardiac c-kit(+) cells. By targeting the c-kit locus with multiple reporter genes in mice, we find that c-kit expression rarely co-localizes with the expression of the cardiac progenitor and myogenic marker Nkx2.5, or that of the myocardial marker, cardiac troponin T (cTnT). Instead, c-kit predominantly labels a cardiac endothelial cell population in developing and adult hearts. After acute cardiac injury, c-kit(+) cells retain their endothelial identity and do not become myogenic progenitors or cardiomyocytes. Thus, our work strongly suggests that c-kit(+) cells in the murine heart are endothelial cells and not CSCs.

Indexed as

AnimalsCell ProliferationCells, CulturedEndothelial CellsFemaleHomeobox Protein Nkx-2.5Homeodomain ProteinsHumansMaleMiceMyocardial InfarctionMyocytes, CardiacProto-Oncogene Proteins c-kitStem CellsTranscription FactorsHomeobox Protein Nkx-2.5Homeodomain ProteinsNkx2-5 protein, mouseProto-Oncogene Proteins c-kitTranscription Factors

Identifiers

PMID26515110
PMCPMC4846318

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.