ArticleJournal of bacteriology1989
Localization and assembly into the Escherichia coli envelope of a protein required for entry of colicin A.
Article in Journal of bacteriology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 56 citations in OpenAlex.
- Similarities and Differences between Colicin and Filamentous Phage Uptake by Bacterial Cells.EcoSal Plus · 2019Review
- Mutations in Escherichia coli ExbB transmembrane domains identify scaffolding and signal transduction functions and exclude participation in a proton pathway.Journal of bacteriology · 2013Article
- Detection and functional characterization of a 215 amino acid N-terminal extension in the Xanthomonas type III effector XopD.PloS one · 2010Article
- Colicin biology.Microbiology and molecular biology reviews : MMBR · 2007Review
- Salmonella enterica serovar typhimurium resistance to bile: identification and characterization of the tolQRA cluster.Journal of bacteriology · 2002Article
- Role of TolR N-terminal, central, and C-terminal domains in dimerization and interaction with TolA and tolQ.Journal of bacteriology · 1999Article
- Maturation and localization of the TolB protein required for colicin import.Journal of bacteriology · 1994Article
- Translocation of DNA across bacterial membranes.Microbiological reviews · 1994Review
- Membrane topology and mutational analysis of the TolQ protein of Escherichia coli required for the uptake of macromolecules and cell envelope integrity.Journal of bacteriology · 1994Article
- Membrane topology of the Escherichia coli TolR protein required for cell envelope integrity.Journal of bacteriology · 1993Article
- Membrane topologies of the TolQ and TolR proteins of Escherichia coli: inactivation of TolQ by a missense mutation in the proposed first transmembrane segment.Journal of bacteriology · 1993Article
- Role of tol genes in cloacin DF13 susceptibility of Escherichia coli K-12 strains expressing the cloacin DF13-aerobactin receptor IutA.Journal of bacteriology · 1993Article
- Membrane topography of ColE1 gene products: the immunity protein.Journal of bacteriology · 1991Article
- Transport of iron across the outer membrane.Biology of metals · 1991Review
- Characterization of ompF domains involved in Escherichia coli K-12 sensitivity to colicins A and N.Journal of bacteriology · 1990Article
- The mechanism of secretion of hemolysin and other polypeptides from gram-negative bacteria.Journal of bioenergetics and biomembranes · 1990Review
- Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli.Journal of bacteriology · 1989Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutations in tolQ, previously designated fii, render cells tolerant to high concentrations of colicin A. In addition, a short deletion in the amino-terminal region of colicin A (amino acid residues 16 to 29) prevents its lethal action, although this protein can still bind the receptor and forms channels in planar lipid bilayers in vitro. These defects in translocation across the outer membrane in the tolQ cells or the colicin A mutant cannot be bypassed by osmotic shock. The TolQ protein, which is constitutively expressed at a low level, was studied in recombinant plasmid constructs allowing the expression of various TolQ fusion proteins under the control of the inducible caa promoter. The TolQ protein was thus "tagged" with an epitope from the colicin A protein for which a monoclonal antibody is available. A fusion protein containing the entire TolQ protein plus the 30 N-terminal residues of colicin A was shown to complement the tolQ mutation. Pulse-chase labeling followed by gradient fractionation indicated that the bulk of the overproduced fusion protein was rapidly incorporated into the inner membrane, with small amounts localized to regions corresponding to the attachment sites between inner and outer membranes and to the outer membrane itself. However, most of the protein was rapidly degraded, leaving only that localized to the attachment sites and the outer membrane remaining at very late times of chase.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.