Evidence map›Paper›PMID 26513671›Full record

ArticleNature protocols2015

Human norovirus culture in B cells.

Melissa K Jones, Katrina R Grau, Veronica Costantini, Abimbola O Kolawole, Miranda de Graaf, Pamela Freiden, Christina L Graves, Marion Koopmans, Shannon M Wallet, Scott A Tibbetts and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers.

0numbers the graph read from it
0cells of the map it votes in
141citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

141 citing papers in PubMed.

  1. Review
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81 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Melissa K JonesDepartment of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, USA.
Katrina R GrauDepartment of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, USA.
Veronica CostantiniCenters for Disease Control and Prevention, Atlanta, Georgia, USA.
Abimbola O KolawoleDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.
Miranda de GraafDepartment of Viroscience; Erasmus Medical Center, Rotterdam, The Netherlands.
Pamela FreidenDepartment of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Christina L GravesDepartment of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida, USA.
Marion KoopmansDepartment of Viroscience; Erasmus Medical Center, Rotterdam, The Netherlands.
Shannon M WalletDepartment of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida, USA.
Scott A TibbettsDepartment of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, USA.
Stacey Schultz-CherryDepartment of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Christiane E WobusDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.
Jan VinjéCenters for Disease Control and Prevention, Atlanta, Georgia, USA.
Stephanie M KarstDepartment of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, USA.

Funding

BASIC MICROBIOLOGY &INFECTIOUS DISEASEST32AI007110 · NIAID · UNIVERSITY OF FLORIDA · PI Stephanie M Karst · 1985 to 2026
$4.3M
Role of Intestinal Bacteria in Human Norovirus InfectionR01AI116892 · NIAID · UNIVERSITY OF FLORIDA · PI KARST, STEPHANIE M · 2015 to 2019
$1.8M
Mechanisms of Norovirus EntryR01AI080611 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WOBUS, CHRISTIANE · 2009 to 2012
$1.5M
Subversion of gastrointestinal host defenses by norovirusR21AI103961 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WOBUS, CHRISTIANE · 2013 to 2014
$428k
NIAID NIH HHS 1R01AI116892NIAID NIH HHS R01 AI080611NIAID NIH HHS R01 AI116892NIAID NIH HHS R21 AI103961NIAID NIH HHS T32 AI007110
6 · The paper itself

Abstract

Human noroviruses (HuNoVs) are a leading cause of foodborne disease and severe childhood diarrhea, and they cause a majority of the gastroenteritis outbreaks worldwide. However, the development of effective and long-lasting HuNoV vaccines and therapeutics has been greatly hindered by their uncultivability. We recently demonstrated that a HuNoV replicates in human B cells, and that commensal bacteria serve as a cofactor for this infection. In this protocol, we provide detailed methods for culturing the GII.4-Sydney HuNoV strain directly in human B cells, and in a coculture system in which the virus must cross a confluent epithelial barrier to access underlying B cells. We also describe methods for bacterial stimulation of HuNoV B cell infection and for measuring viral attachment to the surface of B cells. Finally, we highlight variables that contribute to the efficiency of viral replication in this system. Infection assays require 3 d and attachment assays require 3 h. Analysis of infection or attachment samples, including RNA extraction and RT-qPCR, requires ∼6 h.

Indexed as

B-LymphocytesCaliciviridae InfectionsCell Culture TechniquesCell LineCoculture TechniquesHumansNorovirusReal-Time Polymerase Chain ReactionVirus CultivationVirus InternalizationVirus Replication

Identifiers

PMID26513671
PMCPMC4689599

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.