ReviewNature methods2015
Focused ion beams in biology.
Review in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
96 citing papers in PubMed.
- Vertically Aligned Nanopillar Electrodes: Engineered Interfaces for Electrophysiology and Cell-Electrode Coupling.Small methods · 2026Review
- Electron microscopy-based three-dimensional subcellular imaging of plant male gametophyte.Journal of integrative plant biology · 2026Review
- Landscape expansion microscopy reveals interactions between membrane and phase-separated organelles.The Journal of cell biology · 2026Article
- Intravital single-molecule imaging reveals cytoskeletal turnover as a driver of membrane remodeling in live animals.bioRxiv : the preprint server for biology · 2026Article
- The Freiburg framework for multimodalFrontiers in synaptic neuroscience · 2026Article
- Three-dimensional visualization of tip-vesicles in growing pollen tubes by electron tomography.Science China. Life sciences · 2026Article
- Volume electron microscopy in axon regeneration research: insights into mitochondria, endoplasmic reticulum, and membrane contacts.Frontiers in neural circuits · 2026Review
- Landscape Expansion Microscopy Reveals Interactions between Membrane and Phase-Separated Organelles.bioRxiv : the preprint server for biology · 2025Article
- Cryo-SEM and large volume FIB-SEM of Arabidopsis cotyledons: Degradation of lipid bodies, biogenesis of glyoxysomes and reorganisation of organelles during germination.Journal of microscopy · 2025Article
- Biological volume EM with focused Ga ion beam depends on formation of radiation-resistant Ga-rich layer at block face.Research square · 2025Article
- Review
- A comprehensive overview of focused ion beam-scanning electron microscopy (FIB-SEM) applications for the evaluation of outer retina.Frontiers in cell and developmental biology · 2025Review
- Plume divergence characterization for electric propulsion via standard deviation and emittance.Journal of electric propulsion · 2025Article
- Coordination of force-generating actin-based modules stabilizes and remodels membranes in vivo.The Journal of cell biology · 2024Article
- Review
- The application and development of electron microscopy for three-dimensional reconstruction in life science: a review.Cell and tissue research · 2024Review
- Dynamic changes in endoplasmic reticulum morphology and its contact with the plasma membrane in motor neurons in response to nerve injury.Cell and tissue research · 2024Article
- In vitro co-culture models for studying organoids-macrophages interaction: the golden technology of cancer immunotherapy.American journal of cancer research · 2024Review
- Sample preparation and data collection for serial block face scanning electron microscopy of mammalian cell monolayers.PloS one · 2024Article
- CDK2 inhibition disorders centrosome stoichiometry and alters cellular outcomes in aneuploid cancer cells.Cancer biology & therapy · 2023Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
A quiet revolution is under way in technologies used for nanoscale cellular imaging. Focused ion beams, previously restricted to the materials sciences and semiconductor fields, are rapidly becoming powerful tools for ultrastructural imaging of biological samples. Cell and tissue architecture, as preserved in plastic-embedded resin or in plunge-frozen form, can be investigated in three dimensions by scanning electron microscopy imaging of freshly created surfaces that result from the progressive removal of material using a focused ion beam. The focused ion beam can also be used as a sculpting tool to create specific specimen shapes such as lamellae or needles that can be analyzed further by transmission electron microscopy or by methods that probe chemical composition. Here we provide an in-depth primer to the application of focused ion beams in biology, including a guide to the practical aspects of using the technology, as well as selected examples of its contribution to the generation of new insights into subcellular architecture and mechanisms underlying host-pathogen interactions.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.