ArticleBiomaterials2016
Matrix metalloproteinase-20 mediates dental enamel biomineralization by preventing protein occlusion inside apatite crystals.
Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Integrative multi-omics and bioinformatic analysis ofThe Japanese dental science review · 2026Review
- Review
- Biomineral-Based Composite Materials in Regenerative Medicine.International journal of molecular sciences · 2024Review
- Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature.Exploration (Beijing, China) · 2023Review
- High-resolution Raman spectroscopy reveals compositional differences between pigmented incisor enamel and unpigmented molar enamel in Rattus norvegicus.Scientific reports · 2023Article
- The current regenerative medicine approaches of craniofacial diseases: A narrative review.Frontiers in cell and developmental biology · 2023Review
- Mesoscale structural gradients in human tooth enamel.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Enamel biomineralization under the effects of indomethacin and celecoxib non-steroidal anti-inflammatory drugs.Scientific reports · 2022Article
- Advanced materials for enamel remineralization.Frontiers in bioengineering and biotechnology · 2022Review
- The Skeleton and Biomineralization Mechanism as Part of the Innate Immune System of Stony Corals.Frontiers in immunology · 2022Review
- Advances in biomineralization-inspired materials for hard tissue repair.International journal of oral science · 2021Review
- The association between genetic polymorphisms in matrix metalloproteinases and caries experience.Clinical oral investigations · 2021Article
- Biomimetic mineralisation systems for in situ enamel restoration inspired by amelogenesis.Journal of materials science. Materials in medicine · 2021Review
- Transcriptomic analysis of shell repair and biomineralization in the blue mussel, Mytilus edulis.BMC genomics · 2021Article
- Challenges of Engineering Biomimetic Dental and Paradental Tissues.Tissue engineering and regenerative medicine · 2020Review
- A comparison of proteomic, genomic, and osteological methods of archaeological sex estimation.Scientific reports · 2020Article
- Proteolysis by MMP20 Prevents Aberrant Mineralization in Secretory Enamel.Journal of dental research · 2019Article
- Peptide Self-Assembly into Hydrogels for Biomedical Applications Related to Hydroxyapatite.Gels (Basel, Switzerland) · 2019Review
- Enamel biomimetics-fiction or future of dentistry.International journal of oral science · 2019Review
- The evaluation of zinc and copper content in tooth enamel without any pathological changes - an in vitro study.International journal of nanomedicine · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Reconstruction of enamel-like materials is a central topic of research in dentistry and material sciences. The importance of precise proteolytic mechanisms in amelogenesis to form a hard tissue with more than 95% mineral content has already been reported. A mutation in the Matrix Metalloproteinase-20 (MMP-20) gene results in hypomineralized enamel that is thin, disorganized and breaks from the underlying dentin. We hypothesized that the absence of MMP-20 during amelogenesis results in the occlusion of amelogenin in the enamel hydroxyapatite crystals. We used spectroscopy and electron microscopy techniques to qualitatively and quantitatively analyze occluded proteins within the isolated enamel crystals from MMP-20 null and Wild type (WT) mice. Our results showed that the isolated enamel crystals of MMP-20 null mice had more organic macromolecules occluded inside them than enamel crystals from the WT. The crystal lattice arrangements of MMP-20 null enamel crystals analyzed by High Resolution Transmission Electron Microscopy (HRTEM) were found to be significantly different from those of the WT. Raman studies indicated that the crystallinity of the MMP-20 null enamel crystals was lower than that of the WT. In conclusion, we present a novel functional mechanism of MMP-20, specifically prevention of unwanted organic material entrapped in the forming enamel crystals, which occurs as the result of precise amelogenin cleavage. MMP-20 action guides the growth morphology of the forming hydroxyapatite crystals and enhances their crystallinity. Elucidating such molecular mechanisms can be applied in the design of novel biomaterials for future clinical applications in dental restoration or repair.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.