ArticleJournal of clinical laboratory analysis2016
Development and Validation of an Enzymatic Method for Total Cholesterol Analysis Using Whole Blood Spot.
Article in Journal of clinical laboratory analysis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Dried Blood Spots to Assess Cardiovascular-Kidney-Metabolic Health.Journal of the American Heart Association · 2025Pooled it
- A perinatal coparenting intervention: Effects of a randomized trial on parent cardiometabolic risk and self-reported health.Biological psychology · 2023Trial
- Longitudinal associations between father- and mother-child interactions, coparenting, and child cardiometabolic health.Health psychology : official journal of the Division of Health Psychology, American Psychological Association · 2026Article
- HDL-C as a potential mediator between serum 25(OH)D and the angiographic severity of coronary artery disease: a single-center cross-sectional study.Clinics (Sao Paulo, Brazil) · 2026Article
- Metabolomic and biochemical evaluation of linalool and methyl eugenol as natural alternatives to varenicline in nicotine-induced metabolic dysfunction.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Investigating the dietary inflammatory index and pro-inflammatory immune dynamics: insights from a cross-sectional study of severe vs. non-severe coronary artery disease patients.European journal of medical research · 2025Article
- Ultra-processed foods and coronary artery disease severity: a cross-sectional study of at-risk normal-weight and overweight patients undergoing elective angiography.Journal of health, population, and nutrition · 2025Article
- Fertility protective effects of Brillantaisia patula leaf extract against cyclophosphamide-induced ovarian damage in Wistar rats.BMC biotechnology · 2024Article
- Total Cholesterol Determination Accuracy in Dried Blood Spots.Diagnostics (Basel, Switzerland) · 2024Article
- A pre-clinical study to investigate the anti-diabetic potential ofJournal of diabetes and metabolic disorders · 2023Article
- Insulin resistance surrogate markers and risk of hyperuricemia among patients with and without coronary artery disease: a cross-sectional study.Frontiers in nutrition · 2023Article
- Analytical Validation of Familial Hypercholesterolemia Biomarkers in Dried Blood Spots.International journal of neonatal screening · 2022Article
- Effect of Green Synthesized Iron Oxide Nanoparticles Using Spinach Extract on Triton X-100-Induced Atherosclerosis in Rats.Biochemistry research international · 2022Article
- Non-Lethal Blood Sampling of Fish in the lab and Field With Methods for Dried Blood Plasma Spot Omic Analyses.Frontiers in genetics · 2022Article
- Article
- Combining lipidomics and machine learning to measure clinical lipids in dried blood spots.Metabolomics : Official journal of the Metabolomic Society · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHigh serum cholesterol represents a risk factor for cardiovascular disease. This study aims to quantify total cholesterol in dried blood spot (DBS) by direct enzymatic method.
methodsThree hundred seventeen blood samples with serum cholesterol level ranging from 81 to 337 mg/dl were collected. DBS were manually prepared, cholesterol was extracted using methanol and analyzed by a manual enzymatic method. DBS cholesterol method was validated for imprecision and extraction efficacy. DBS cholesterol values were correlated (training test) with serum values measured by automated enzymatic method (reference method). The obtained correlation was used for predicting serum cholesterol from DBS analysis of a new sample group (validation test, n = 58).
resultsWithin-day and between-day coefficient of variation (CV%) were lower than 7.69 and 6.32, respectively. Residual cholesterol in DBS after extraction was 16%. DBS cholesterol and serum cholesterol showed a linear correlation (slope = 0.5217; r = 0.9139) and a bias of -28%. Furthermore, DBS cholesterol values of validation test (n = 58), converted using the training test correlation, were not statistically different compared to the corresponding plasma values (P = 0.9487), and the comparison by Passing and Bablok showed a linear regression with a slope of 1.068 (r = 0.611) and a bias of -0.22%.
conclusionsThe results show that this enzymatic method is suitable to analyze cholesterol in DBS and it could be automated and used for population screening of total blood cholesterol.
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