ArticleBMC genomics2015
Identification of miRNAs with potential roles in regulation of anther development and male-sterility in 7B-1 male-sterile tomato mutant.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Identification of differentially expressed miRNAs between male sterile and fertile floral buds in watermelon (3 Biotech · 2024Article
- Heat-responsive microRNAs participate in regulating the pollen fertility stability of CMS-D2 restorer line under high-temperature stress.Biological research · 2023Article
- Genome-wide identification of Sclerotinia sclerotiorum small RNAs and their endogenous targets.BMC genomics · 2023Article
- Polar auxin transport modulates early leaf flattening.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The miR393-Target Module Regulates Plant Development and Responses to Biotic and Abiotic Stresses.International journal of molecular sciences · 2022Review
- miR390 family of Cymbidium goeringii is involved in the development of reproductive organs in transgenic Arabidopsis.BMC plant biology · 2022Article
- Modifying Anthocyanins Biosynthesis in Tomato Hairy Roots: A Test Bed for Plant Resistance to Ionizing Radiation and Antioxidant Properties in Space.Frontiers in plant science · 2022Article
- fIdentification of B. napus small RNAs responsive to infection by a necrotrophic pathogen.BMC plant biology · 2021Article
- Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the MiR156, MiR5488 and MiR399 are Involved in the Regulation of Male Sterility in PTGMS Rice.International journal of molecular sciences · 2021Article
- Article
- Blocking miR530 Improves Rice Resistance, Yield, and Maturity.Frontiers in plant science · 2021Article
- Noncoding RNAs: functional regulatory factors in tomato fruit ripening.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Article
- Functional Characterization of microRNA171 Family in Tomato.Plants (Basel, Switzerland) · 2019Article
- Gene characterization and molecular pathway analysis of reverse thermosensitive genic male sterility in eggplant (Horticulture research · 2019Article
- Novel and Conserved miRNAs Among Brazilian Pine and Other Gymnosperms.Frontiers in genetics · 2019Article
- Profiling miRNA expression in photo-thermo-sensitive male genic sterility line (PTGMS) PA64S under high and low temperature.Plant signaling & behavior · 2019Article
- Tapetum-Dependent Male Meiosis Progression in Plants: Increasing Evidence Emerges.Frontiers in plant science · 2019Review
- Article
- MicroRNA profiling analysis of developing berries for 'Kyoho' and its early-ripening mutant during berry ripening.BMC plant biology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe 7B-1 tomato line (Solanum lycopersicum cv. Rutgers) is a photoperiod-sensitive male-sterile mutant, with potential application in hybrid seed production. Small RNAs (sRNAs) in tomato have been mainly characterized in fruit development and ripening, but none have been studied with respect to flower development and regulation of male-sterility. Using sRNA sequencing, we identified miRNAs that are potentially involved in anther development and regulation of male-sterility in 7B-1 mutant.
resultsTwo sRNA libraries from 7B-1 and wild type (WT) anthers were sequenced and thirty two families of known miRNAs and 23 new miRNAs were identified in both libraries. MiR390, miR166, miR159 were up-regulated and miR530, miR167, miR164, miR396, miR168, miR393, miR8006 and two new miRNAs, miR#W and miR#M were down-regulated in 7B-1 anthers. Ta-siRNAs were not differentially expressed and likely not associated with 7B-1 male-sterility. miRNA targets with potential roles in anther development were validated using 5'-RACE. QPCR analysis showed differential expression of miRNA/target pairs of interest in anthers and stem of 7B-1, suggesting that they may regulate different biological processes in these tissues. Expression level of most miRNA/target pairs showed negative correlation, except for few. In situ hybridization showed predominant expression of miR159, GAMYBL1, PMEI and cystatin in tapetum, tetrads and microspores.
conclusionOverall, we identified miRNAs with potential roles in anther development and regulation of male-sterility in 7B-1. A number of new miRNAs were also identified from tomato for the first time. Our data could be used as a benchmark for future studies of the molecular mechanisms of male-sterility in other crops.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.