ArticleBMC genomics2015
De novo transcriptome sequencing in Bixa orellana to identify genes involved in methylerythritol phosphate, carotenoid and bixin biosynthesis.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Chromosome-scale, single haploid assembly of achiote (Bixa orellana).BMC genomic data · 2026Article
- Genome-wide identification and characterization of the carotenoid cleavage dioxygenase gene family inFrontiers in plant science · 2026Article
- Water-soluble carotenoid: focused on natural carotenoid crocin.Food science and biotechnology · 2025Review
- Gene structure and potential regulation of the lycopene cyclase genes inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Dynamics of annatto pigment synthesis and accumulation in seeds of Bixa orellana L. revealed by integrated chemical, anatomical, and RNA-Seq analyses.Protoplasma · 2023Article
- Gene expression profile during seed development ofFrontiers in plant science · 2023Article
- Therapeutic potential of bixin on inflammation: a mini review.Frontiers in nutrition · 2023Review
- Gene expression analysis during the fruit development in dehiscent and indehiscentPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Old roads revisited: Discovery of a crocin synthesis enzyme.Plant physiology · 2022Article
- Heterologous expression of Bixa orellana cleavage dioxygenase 4-3 drives crocin but not bixin biosynthesis.Plant physiology · 2022Article
- IdentifyingFrontiers in plant science · 2022Article
- Carotenoids and Apocarotenoids in Planta: Their Role in Plant Development, Contribution to the Flavour and Aroma of Fruits and Flowers, and Their Nutraceutical Benefits.Plants (Basel, Switzerland) · 2021Review
- De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production.Protoplasma · 2019Article
- Article
- The Specialized Roles in Carotenogenesis and Apocarotenogenesis of the Phytoene Synthase Gene Family in Saffron.Frontiers in plant science · 2019Article
- Article
- Apocarotenoids: Emerging Roles in Mammals.Annual review of nutrition · 2018Article
- The biotechnology (genetic transformation and molecular biology) of Bixa orellana L. (achiote).Planta · 2018Review
- Expression and Interaction Analysis among Saffron ALDHs and Crocetin Dialdehyde.International journal of molecular sciences · 2018Article
- Selection and validation of reference genes for quantitative gene expression analyses in various tissues and seeds at different developmental stages inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBixin or annatto is a commercially important natural orange-red pigment derived from lycopene that is produced and stored in seeds of Bixa orellana L. An enzymatic pathway for bixin biosynthesis was inferred from homology of putative proteins encoded by differentially expressed seed cDNAs. Some activities were later validated in a heterologous system. Nevertheless, much of the pathway remains to be clarified. For example, it is essential to identify the methylerythritol phosphate (MEP) and carotenoid pathways genes.
resultsIn order to investigate the MEP, carotenoid, and bixin pathways genes, total RNA from young leaves and two different developmental stages of seeds from B. orellana were used for the construction of indexed mRNA libraries, sequenced on the Illumina HiSeq 2500 platform and assembled de novo using Velvet, CLC Genomics Workbench and CAP3 software. A total of 52,549 contigs were obtained with average length of 1,924 bp. Two phylogenetic analyses of inferred proteins, in one case encoded by thirteen general, single-copy cDNAs, in the other from carotenoid and MEP cDNAs, indicated that B. orellana is closely related to sister Malvales species cacao and cotton. Using homology, we identified 7 and 14 core gene products from the MEP and carotenoid pathways, respectively. Surprisingly, previously defined bixin pathway cDNAs were not present in our transcriptome. Here we propose a new set of gene products involved in bixin pathway.
conclusionThe identification and qRT-PCR quantification of cDNAs involved in annatto production suggest a hypothetical model for bixin biosynthesis that involve coordinated activation of some MEP, carotenoid and bixin pathway genes. These findings provide a better understanding of the mechanisms regulating these pathways and will facilitate the genetic improvement of B. orellana.
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