ArticleJournal of anatomy2016
Histopathological analysis of the olfactory epithelium of zebrafish (Danio rerio) exposed to sublethal doses of urea.
Article in Journal of anatomy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Novel mechanisms of chemosensory adaptation to the cave environment.bioRxiv : the preprint server for biology · 2026Article
- Mouse Gnal transcripts and transcriptomics in isolated dystonia.Research square · 2025Article
- Impact of urea nitrogen fertilizer on the physiology, behavior, and histology of juvenile rainbow trout.Fish physiology and biochemistry · 2025Article
- Olfactory Impairment and Recovery in Zebrafish (Biology · 2025Article
- Intranasal delivery of SARS-CoV-2 spike protein is sufficient to cause olfactory damage, inflammation and olfactory dysfunction in zebrafish.Brain, behavior, and immunity · 2022Article
- Localization of BDNF and Calretinin in Olfactory Epithelium and Taste Buds of Zebrafish (International journal of molecular sciences · 2022Article
- Gross morphology, histology, and ultrastructure of the olfactory rosette of a critically endangered indicator species, the Delta Smelt, Hypomesus transpacificus.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2021Article
- A comprehensive structural, lectin and immunohistochemical characterization of the zebrafish olfactory system.Scientific reports · 2021Article
- The Olfactory System of Zebrafish as a Model for the Study of Neurotoxicity and Injury: Implications for Neuroplasticity and Disease.International journal of molecular sciences · 2019Review
- Exposure to Zinc Sulfate Results in Differential Effects on Olfactory Sensory Neuron Subtypes in Adult Zebrafish.International journal of molecular sciences · 2016Article
- The Biological Roles of Urea: A Review of Preclinical Studies.Indian journal of nephrologyReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chronic renal disease is known to alter olfactory function, but the specific changes induced in olfactory organs during this process remain unclear. Of the uraemic toxins generated during renal disease, high levels of urea are known to induce hyposmic conditions. In this study, the effects of environmental exposure to elevated concentrations of urea (7, 13.5 and 20 g L(-1)) on the sensory mucosa of zebrafish in acute toxicity and chronic toxicity tests were described. It was observed that lamellae maintained structural integrity and epithelial thickness was slightly reduced, but only following exposure to the highest concentrations of urea. Pan-neuronal labelling with anti-Hu revealed a negative correlation with levels of urea, leading to investigation of whether distinct neuronal subtypes were equally sensitive. Using densitometric analysis of immunolabelled tissues, numbers of Gα olf-, TRPC2- and TrkA-expressing cells were compared, representing ciliated, microvillous and crypt neurons, respectively. The three neuronal subpopulations responded differently to increasing levels of urea. In particular, crypt cells were more severely affected than the other cell types, and Gα olf-immunoreactivity was found to increase when fish were exposed to low doses of urea. It can be concluded that exposure to moderate levels of urea leads to sensory toxicity directly affecting olfactory organs, in accordance with the functional olfactometric measurements previously reported in the literature.
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Registered trials
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