ArticleScientific reports2015
Production of reproductively sterile fish by a non-transgenic gene silencing technology.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 73 citations in OpenAlex.
- Bath-Immersion Delivery of nanos-MO-Vivo During Embryogenesis Perturbs Gonadal Development in the Pacific Oyster (Crassostrea gigas).Marine biotechnology (New York, N.Y.) · 2026Article
- Primordial germ cell specification and early developmental cell states in Pacific oyster.BMC genomics · 2025Article
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- Migration of primordial germ cells and their relationship of PGCs with sex development in transgenic germline-specific fluorescent freshwater angelfish (Pterophyllum scalare).Scientific reports · 2025Article
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- Cellular remodeling and JAK inhibition promote zygotic gene expression in the Ciona germline.EMBO reports · 2024Article
- Insights into Early Ontogenesis ofAnimals : an open access journal from MDPI · 2023Article
- Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of theFrontiers in genetics · 2023Article
- Impact of germ cell ablation on the activation of the brain-pituitary-gonadal axis in precocious Atlantic salmon (Salmo salar L.) males.Molecular reproduction and development · 2022Article
- Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture-A Practical Review.Animals : an open access journal from MDPI · 2022Review
- The Piwil1 N domain is required for germ cell survival in Atlantic salmon.Frontiers in cell and developmental biology · 2022Article
- A Novel Allotriploid Hybrid Derived From Female Goldfish × Male Bleeker's Yellow Tail.Frontiers in genetics · 2022Article
- Article
- A transgenic DND1GFP fusion allele reports in vivo expression and RNA-binding targets in undifferentiated mouse germ cells†.Biology of reproduction · 2021Article
- Maintenance management and eradication of established aquatic invaders.Hydrobiologia · 2021Review
- Fish reproductive biology - Reflecting on five decades of fundamental and translational research.General and comparative endocrinology · 2021Review
- Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon.Scientific reports · 2020Article
- Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon.BMC genomics · 2020Article
- Article
- Repressible Transgenic Sterilization in Channel Catfish, Ictalurus punctatus, by Knockdown of Primordial Germ Cell Genes with Copper-Sensitive Constructs.Marine biotechnology (New York, N.Y.) · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a novel bath-immersion technology to produce large numbers of infertile fish. As seafood consumption shifts from fishery harvests towards artificially propagated species, optimization of aquaculture practices will be necessary to maximize food production and minimize ecological impact. Farming infertile fish is the most effective genetic-containment strategy to support the development of environmentally-responsible aquaculture. We discovered that a molecular transporter, Vivo, can effectively carry the Morpholino oligomer (MO) across the chorion, enter the embryo and reach target cells. Vivo-conjugated MO against zebrafish deadend (dnd-MO-Vivo) effectively caused primordial germ cell mis-migration and differentiation into somatic cells, which resulted in generation of infertile fish. Optimal conditions were achieved when embryos, immediately after fertilization, were immersed with dnd-MO-Vivo at the initial concentration of either 60 or 40 μM followed by a lower serially diluted concentration. Under these conditions, 100% induced sterility was achieved even when the total immersion time was reduced from 24 to 5 hours. In 8 independent experiments, 736 adults developed from these conditions were all found to be infertile fish that possessed minimally-developed gonads that lacked any gametes. The results demonstrate that dnd-MO-Vivo bath immersion is an effective strategy to produce infertile fish without introducing transgenic modifications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.