ArticleApplied immunohistochemistry & molecular morphology : AIMM2017
Optimized Protocol of Methanol Treatment for Immunofluorescent Staining in Fixed Brain Slices.
Article in Applied immunohistochemistry & molecular morphology : AIMM, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Assessment of different preservation techniques for human skeletal muscle biopsy samples: A comparative method study on freeze-drying, RNAlater, and RNAlater-ICE.Physiological reports · 2025Article
- Alternative Brain Slice-on-a-Chip for Organotypic Culture and Effective Fluorescence Injection Testing.International journal of molecular sciences · 2022Article
- Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study.Frontiers in neuroanatomy · 2022Article
- Potential evidence of peripheral learning and memory in the arms of dwarf cuttlefish, Sepia bandensis.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2021Article
- Verification of the Cross Immunoreactivity of A60, a Mouse Monoclonal Antibody against Neuronal Nuclear Protein.Frontiers in neuroanatomy · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
We optimized methanol treatment in paraformaldehyde-fixed slices for immunofluorescent staining of ependymal basal bodies in brain ventricles. As 100% methanol induced severe deformations to the slices (including rolling and folding over), we tried to decrease methanol concentration. We found that 33.3% to 75% methanol could result in ideal immunostaining of basal bodies without inducing obvious deformations. Instead of treating slices at -20°C (without proper cryoprotection measurements) as suggested in previous studies, we carried out methanol treatment at room temperature. Our modified protocol can not only raise immunostaining efficiency in tissue slices, it may also prevent potential freezing damages to the samples.
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Registered trials
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