Evidence map›Paper›PMID 26509875›Full record

ArticleMucosal immunology2016

Salivary IgA from the sublingual compartment as a novel noninvasive proxy for intestinal immune induction.

A Aase, H Sommerfelt, L B Petersen, M Bolstad, R J Cox, N Langeland, A B Guttormsen, H Steinsland, S Skrede, P Brandtzaeg

Registry-linked trialOpen access · bronzeAbstract readComparative Study
PubMed Publisher
In one paragraph

Article in Mucosal immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02870751 (Developing A Human Challenge Model For Evaluating Vaccines Against ST-producing Enterotoxigenic Escherichia Coli), which is not on this map. Cited by 28 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 1 pooled it
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02870751 phase1unknown statusnot on this map

Developing A Human Challenge Model For Evaluating Vaccines Against ST-producing Enterotoxigenic Escherichia Coli

TypeinterventionalSponsorUniversity of BergenRan2016 to 2019Enrolled30ConditionsDiarrhea, InfantileArmsTW11681 or TW10722
3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.

  1. Pooled it
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  5. Review
  6. Review
  7. Article
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  16. Review
  17. Mucosal vaccines - fortifying the frontiers.Nature reviews. Immunology · 2022
    Review
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  19. Observational
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

A AaseDivision of Infectious Disease Control, Norwegian Institute of Public Health, Oslo, Norway.
H SommerfeltCenter for Intervention Science in Maternal and Child Health and Centre for International health, Centre for International Health, University of Bergen, Bergen, Norway.
L B PetersenDivision of Infectious Disease Control, Norwegian Institute of Public Health, Oslo, Norway.
M BolstadDivision of Infectious Disease Control, Norwegian Institute of Public Health, Oslo, Norway.
R J CoxDepartment of Clinical Science, University of Bergen, Bergen, Norway.
N LangelandDepartment of Clinical Science, University of Bergen, Bergen, Norway.
A B GuttormsenDepartment of Anesthesia and Intensive Care, Haukeland University Hospital, Bergen, Norway.
H SteinslandCenter for Intervention Science in Maternal and Child Health and Centre for International health, Centre for International Health, University of Bergen, Bergen, Norway.
S SkredeDepartment of Clinical Science, University of Bergen, Bergen, Norway.
P BrandtzaegLIIPAT, Centre for Immune Regulation, University of Oslo, Oslo, Norway.
Norwegian Institute of Public Health · NOUniversity of Bergen · NOHaukeland University Hospital · NOOslo University Hospital · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Whole-saliva IgA appears like an attractive noninvasive readout for intestinal immune induction after enteric infection or vaccination, but has failed to show consistent correlation with established invasive markers and IgA in feces or intestinal lavage. For reference, we measured antibodies in samples from 30 healthy volunteers who were orally infected with wild-type enterotoxigenic Escherichia coli. The response against these bacteria in serum, lavage, and lymphocyte supernatants (antibody-in-lymphocyte-supernatant, ALS) was compared with that in targeted parotid and sublingual/submandibular secretions. Strong correlation occurred between IgA antibody levels against the challenge bacteria in sublingual/submandibular secretions and in lavage (r=0.69, P<0.0001) and ALS (r=0.70, P<0.0001). In sublingual/submandibular secretions, 93% responded with more than a twofold increase in IgA antibodies against the challenge strain, whereas the corresponding response in parotid secretions was only 67% (P=0.039). With >twofold ALS as a reference, the sensitivity of a >twofold response for IgA in sublingual/submandibular secretion was 96%, whereas it was only 67% in the parotid fluid. To exclude that flow rate variations influenced the results, we used albumin as a marker. Our data suggested that IgA in sublingual/submandibular secretions, rather than whole saliva with its variable content of parotid fluid, is a preferential noninvasive proxy for intestinal immune induction.

Indexed as

Antibodies, BacterialBiomarkersCells, CulturedCulture Media, ConditionedEnterotoxigenic Escherichia coliEscherichia coli InfectionsFecesHumansImmunity, MucosalImmunoglobulin AIntestinesLymphocytesParotid GlandSalivaSensitivity and SpecificityAntibodies, BacterialBiomarkersCulture Media, ConditionedImmunoglobulin A

Identifiers

PMID26509875
OpenAlexW2211220962

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.