ArticlePLoS genetics2015
Silencing of X-Linked MicroRNAs by Meiotic Sex Chromosome Inactivation.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Review
- Sex-Biased Expression and Response of microRNAs in Neurological Diseases and Neurotrauma.International journal of molecular sciences · 2024Review
- Meiotic Recognition of Evolutionarily Diverged Homologs: Chromosomal Hybrid Sterility Revisited.Molecular biology and evolution · 2023Article
- A cluster of X-linked miRNAs are de-repressed with age in mouse liver and target growth hormone signaling.Frontiers in aging · 2023Article
- A Hypothesis: Linking Phase Separation to Meiotic Sex Chromosome Inactivation and Sex-Body Formation.Frontiers in cell and developmental biology · 2021Review
- Transcriptomics of Meiosis in the Male Mouse.Frontiers in cell and developmental biology · 2021Review
- Reverse genetics reveals single gene of every candidate on Hybrid sterility, X Chromosome QTL 2 (Hstx2) are dispensable for spermatogenesis.Scientific reports · 2020Article
- Response to: X-linked miR-506 family miRNAs promote FMRP expression in mouse spermatogonia.EMBO reports · 2020Article
- Article
- Genomic Structure ofGenetics · 2019Article
- Evolution of an X-Linked miRNA Family Predominantly Expressed in Mammalian Male Germ Cells.Molecular biology and evolution · 2019Article
- Identification of the X-linked germ cell specific miRNAs (XmiRs) and their functions.PloS one · 2019Article
- SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice.Developmental cell · 2018Article
- The eXceptional nature of the X chromosome.Human molecular genetics · 2018Review
- Interrogating the Functions of PRDM9 Domains in Meiosis.Genetics · 2018Article
- Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.Biology of reproduction · 2018Article
- Sex-biased microRNA expression in mammals and birds reveals underlying regulatory mechanisms and a role in dosage compensation.Genome research · 2017Article
- Intracytoplasmic sperm injection: state of the art in humans.Reproduction (Cambridge, England) · 2017Review
- Zfy genes are required for efficient meiotic sex chromosome inactivation (MSCI) in spermatocytes.Human molecular genetics · 2016Article
- Sex Chromosomes Do It Differently.PLoS biology · 2016Review
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
During the pachytene stage of meiosis in male mammals, the X and Y chromosomes are transcriptionally silenced by Meiotic Sex Chromosome Inactivation (MSCI). MSCI is conserved in therian mammals and is essential for normal male fertility. Transcriptomics approaches have demonstrated that in mice, most or all protein-coding genes on the X chromosome are subject to MSCI. However, it is unclear whether X-linked non-coding RNAs behave in a similar manner. The X chromosome is enriched in microRNA (miRNA) genes, with many exhibiting testis-biased expression. Importantly, high expression levels of X-linked miRNAs (X-miRNAs) have been reported in pachytene spermatocytes, indicating that these genes may escape MSCI, and perhaps play a role in the XY-silencing process. Here we use RNA FISH to examine X-miRNA expression in the male germ line. We find that, like protein-coding X-genes, X-miRNAs are expressed prior to prophase I and are thereafter silenced during pachynema. X-miRNA silencing does not occur in mouse models with defective MSCI. Furthermore, X-miRNAs are expressed at pachynema when present as autosomally integrated transgenes. Thus, we conclude that silencing of X-miRNAs during pachynema in wild type males is MSCI-dependent. Importantly, misexpression of X-miRNAs during pachynema causes spermatogenic defects. We propose that MSCI represents a chromosomal mechanism by which X-miRNAs, and other potential X-encoded repressors, can be silenced, thereby regulating genes with critical late spermatogenic functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.