ArticlePloS one2015
Validation of Reference Genes for Accurate Normalization of Gene Expression in Lilium davidii var. unicolor for Real Time Quantitative PCR.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Genome-Wide Identification of thePlants (Basel, Switzerland) · 2026Article
- Evaluation of Suitable Reference Gene During the Development of Paired or Unpaired FemalePathogens (Basel, Switzerland) · 2025Article
- Epigenetic silencing of callose synthase by VIL1 promotes bud-growth transition in lily bulbs.Nature plants · 2023Article
- An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor.Scientific reports · 2022Article
- Transcriptome profiling reveals key genes in regulation of the tepal trichome development in Lilium pumilum D.C.Plant cell reports · 2021Article
- Full-Length Transcriptome Analysis of theInternational journal of molecular sciences · 2020Article
- Selection of reliable reference genes for gene expression analysis in seeds at different developmental stages and across various tissues in Paeonia ostii.Molecular biology reports · 2019Article
- Selection of reference genes for quantitative real-time PCR analysis of photosynthesis-related genes expression inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Selection of reference genes for flowering pathway analysis in the masting plants, Celmisia lyallii and Chionochloa pallens, under variable environmental conditions.Scientific reports · 2019Article
- Selection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune.BMC molecular biology · 2019Article
- Evaluation of suitable reference genes for qRT-PCR normalization in strawberry (Fragaria × ananassa) under different experimental conditions.BMC molecular biology · 2018Article
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- Validation of Reference Genes for Quantitative Real-Time PCR during Bicolor Tepal Development in Asiatic Hybrid Lilies (Frontiers in plant science · 2017Article
- Validation of reference genes for normalization of gene expression by qRT-PCR in a resveratrol-producing entophytic fungus (Alternaria sp. MG1).AMB Express · 2016Article
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Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lilium is an important commercial market flower bulb. qRT-PCR is an extremely important technique to track gene expression levels. The requirement of suitable reference genes for normalization has become increasingly significant and exigent. The expression of internal control genes in living organisms varies considerably under different experimental conditions. For economically important Lilium, only a limited number of reference genes applied in qRT-PCR have been reported to date. In this study, the expression stability of 12 candidate genes including α-TUB, β-TUB, ACT, eIF, GAPDH, UBQ, UBC, 18S, 60S, AP4, FP, and RH2, in a diverse set of 29 samples representing different developmental processes, three stress treatments (cold, heat, and salt) and different organs, has been evaluated. For different organs, the combination of ACT, GAPDH, and UBQ is appropriate whereas ACT together with AP4, or ACT along with GAPDH is suitable for normalization of leaves and scales at different developmental stages, respectively. In leaves, scales and roots under stress treatments, FP, ACT and AP4, respectively showed the most stable expression. This study provides a guide for the selection of a reference gene under different experimental conditions, and will benefit future research on more accurate gene expression studies in a wide variety of Lilium genotypes.
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