ArticleNature communications2015
Optimized measurements of separations and angles between intra-molecular fluorescent markers.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Separation estimation of two freely rotating dipole emitters near the quantum limit.Physical review. A · 2026Article
- Transport in the brain studied withNeurophotonics · 2025Review
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- Single-molecule fluorescence multiplexing by multi-parameter spectroscopic detection of nanostructured FRET labels.Nature nanotechnology · 2024Article
- PyF2F: a robust and simplified fluorophore-to-fluorophore distance measurement tool for Protein interactions from Imaging Complexes after Translocation experiments.NAR genomics and bioinformatics · 2024Article
- One must reconstitute the functions of interest from purified proteins.Frontiers in physiology · 2024Review
- Calibrating Catalytic DNA Nanostructures for Site-Selective Protein Modification.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Strategies for Controlling the Spatial Orientation of Single Molecules Tethered on DNA Origami Templates Physisorbed on Glass Substrates: Intercalation and Stretching.International journal of molecular sciences · 2022Article
- Article
- Imaging and Localization of Single Emitters near Plasmonic Particles of Different Size, Shape, and Material.The journal of physical chemistry. C, Nanomaterials and interfaces · 2021Article
- Simultaneous orientation and 3D localization microscopy with a Vortex point spread function.Nature communications · 2021Article
- A gain series method for accurate EMCCD calibration.Scientific reports · 2021Article
- Imaging therapeutic peptide transport across intestinal barriers.RSC chemical biology · 2021Review
- A method for EMCCD multiplication gain measurement with comprehensive correction.Scientific reports · 2021Article
- Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells.Science advances · 2020Article
- Nanometer-accuracy distance measurements between fluorophores at the single-molecule level.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Subnanometer localization accuracy in widefield optical microscopy.Light, science & applications · 2018Article
- DNA origami-based shape IDs for single-molecule nanomechanical genotyping.Nature communications · 2017Article
- Single molecule optical measurements of orientation and rotations of biological macromolecules.Methods and applications in fluorescence · 2016Article
- "Calibration-on-the-spot": How to calibrate an EMCCD camera from its images.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
We demonstrate a novel, yet simple tool for the study of structure and function of biomolecules by extending two-colour co-localization microscopy to fluorescent molecules with fixed orientations and in intra-molecular proximity. From each colour-separated microscope image in a time-lapse movie and using only simple means, we simultaneously determine both the relative (x,y)-separation of the fluorophores and their individual orientations in space with accuracy and precision. The positions and orientations of two domains of the same molecule are thus time-resolved. Using short double-stranded DNA molecules internally labelled with two fixed fluorophores, we demonstrate the accuracy and precision of our method using the known structure of double-stranded DNA as a benchmark, resolve 10-base-pair differences in fluorophore separations, and determine the unique 3D orientation of each DNA molecule, thereby establishing short, double-labelled DNA molecules as probes of 3D orientation of anything to which one can attach them firmly.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.